Aldehyde dehydrogenase activity allows reliable EPC enumeration in stored peripheral blood samples

Thomas J. Povsic, Stacie D. Adams, Katherine L. Zavodni, Francine Kelly, Laura G. Melton, Sunil V. Rao, Samer S. Najjar, Robert A. Harrington, Eric D. Peterson

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Interest in the biology of endogenous progenitor cells (EPCs) continues to grow as evidence of their role in vascular repair mounts. EPC enumeration requires specialized laboratory techniques and is performed immediately after sample acquisition, limiting the clinical contexts in which EPC enumeration can be performed and the ability to increase sample sizes through multi-center participation. Methods: We compared the numbers of EPCs enumerated in samples processed immediately after acquisition (n = 36) with EPCs enumerated in specimens stored for 24 hours or after cryopreservation of mononuclear cells (MNC) using two EPC identification strategies: cell surface marker expression (CD133/CD34) and aldehyde dehydrogenase activity (ALDHbr cells). Results: EPCs assessed in fresh samples correlated with EPCs enumerated after whole blood storage (r = 0.699 for CD133+ CD34+ cells, r = 0.880 for ALDHbr cells, P < 0.005 and P < 0.0001, respectively) or mononuclear cryopreservation (r = 0.590 for CD133+CD34+ cells, r = 0.894 for ALDHbr cells, P < 0.0001 for each); however, correlation based on assessment of ALDHbr cells was higher (P < 0.0003 for comparison of correlation coefficients). Initial results from a multi-site clinical trial suggest that EPC enumeration after mononuclear cell cryopreservation is feasible. Conclusion: EPC analysis based on ALDH activity is reproducible, even after extended whole blood storage or MNC cryopreservation.

Original languageEnglish (US)
Pages (from-to)259-265
Number of pages7
JournalJournal of Thrombosis and Thrombolysis
Volume28
Issue number3
DOIs
StatePublished - 2009
Externally publishedYes

Keywords

  • Aldehyde dehydrogenase
  • Atherosclerosis
  • Cell surface marker analysis
  • Clinical trials
  • Endothelial progenitor cell
  • Stem cell

ASJC Scopus subject areas

  • Hematology
  • Cardiology and Cardiovascular Medicine

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