Allelic heterogeneity of HLA-B35 subtypes in different populations as assessed by DNA typing

M. L. Satz, M. Fernandez-Vina, C. G. Del Theiler, Y. C. Marcos, N. Lindel, M. Capucchio, C. Gorodezky, L. Fainboim, P. Stastny

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

HLA-B35, a class I antigen differentially associated to several diseases in different ethnic groups, comprises at least eight alleles which differ among them by one to six amino acids. In the present work a rapid DNA typing procedure was used to investigate the distribution of the various HLA-B35 alleles in different populations. The approach is based on a group-specific PCR amplification of a set of closely related HLA-B alleles sharing a Thr in position 45 of the alpha-1 domain. The amplified DNA was then hybridized to a panel of sequence-specific oligonucleotide (SSO) probes designed to recognize the polymorphic residues in previously reported HLA-B35 subtypes. This methodology was successfully tested in 100 individuals of four different populations, previously typed by serology as HLA-B35, and in six reference panel cells of the 10th International Histocompatibility Workshop. HLA-B(*)3501 was the predominant subtype in all populations. B(*)3502, B(*)3503 and, to a lesser extent B(*)3508, were also found. Among Mexican Mestizos, thirteen individuals had patterns of SSO hybridization suggestive of new B35 alleles. The evolutionary considerations on the different B35 alleles and their extended B35,Cw4 haplotypes are discussed.

Original languageEnglish (US)
Pages (from-to)196-203
Number of pages8
JournalTissue Antigens
Volume46
Issue number3 I
StatePublished - 1995

Fingerprint

HLA-B35 Antigen
DNA Fingerprinting
Alleles
HLA-B Antigens
Population
Histocompatibility Antigens Class I
Histocompatibility
Oligonucleotide Probes
Serology
Ethnic Groups
Oligonucleotides
Haplotypes
Education
Amino Acids
Polymerase Chain Reaction
DNA

Keywords

  • DNA typing
  • Group-specific amplification
  • HLA-B35 allele
  • PCR
  • Transplantation

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Satz, M. L., Fernandez-Vina, M., Del Theiler, C. G., Marcos, Y. C., Lindel, N., Capucchio, M., ... Stastny, P. (1995). Allelic heterogeneity of HLA-B35 subtypes in different populations as assessed by DNA typing. Tissue Antigens, 46(3 I), 196-203.

Allelic heterogeneity of HLA-B35 subtypes in different populations as assessed by DNA typing. / Satz, M. L.; Fernandez-Vina, M.; Del Theiler, C. G.; Marcos, Y. C.; Lindel, N.; Capucchio, M.; Gorodezky, C.; Fainboim, L.; Stastny, P.

In: Tissue Antigens, Vol. 46, No. 3 I, 1995, p. 196-203.

Research output: Contribution to journalArticle

Satz, ML, Fernandez-Vina, M, Del Theiler, CG, Marcos, YC, Lindel, N, Capucchio, M, Gorodezky, C, Fainboim, L & Stastny, P 1995, 'Allelic heterogeneity of HLA-B35 subtypes in different populations as assessed by DNA typing', Tissue Antigens, vol. 46, no. 3 I, pp. 196-203.
Satz ML, Fernandez-Vina M, Del Theiler CG, Marcos YC, Lindel N, Capucchio M et al. Allelic heterogeneity of HLA-B35 subtypes in different populations as assessed by DNA typing. Tissue Antigens. 1995;46(3 I):196-203.
Satz, M. L. ; Fernandez-Vina, M. ; Del Theiler, C. G. ; Marcos, Y. C. ; Lindel, N. ; Capucchio, M. ; Gorodezky, C. ; Fainboim, L. ; Stastny, P. / Allelic heterogeneity of HLA-B35 subtypes in different populations as assessed by DNA typing. In: Tissue Antigens. 1995 ; Vol. 46, No. 3 I. pp. 196-203.
@article{dea42e8deb34403e8a75bb612bacf5c1,
title = "Allelic heterogeneity of HLA-B35 subtypes in different populations as assessed by DNA typing",
abstract = "HLA-B35, a class I antigen differentially associated to several diseases in different ethnic groups, comprises at least eight alleles which differ among them by one to six amino acids. In the present work a rapid DNA typing procedure was used to investigate the distribution of the various HLA-B35 alleles in different populations. The approach is based on a group-specific PCR amplification of a set of closely related HLA-B alleles sharing a Thr in position 45 of the alpha-1 domain. The amplified DNA was then hybridized to a panel of sequence-specific oligonucleotide (SSO) probes designed to recognize the polymorphic residues in previously reported HLA-B35 subtypes. This methodology was successfully tested in 100 individuals of four different populations, previously typed by serology as HLA-B35, and in six reference panel cells of the 10th International Histocompatibility Workshop. HLA-B(*)3501 was the predominant subtype in all populations. B(*)3502, B(*)3503 and, to a lesser extent B(*)3508, were also found. Among Mexican Mestizos, thirteen individuals had patterns of SSO hybridization suggestive of new B35 alleles. The evolutionary considerations on the different B35 alleles and their extended B35,Cw4 haplotypes are discussed.",
keywords = "DNA typing, Group-specific amplification, HLA-B35 allele, PCR, Transplantation",
author = "Satz, {M. L.} and M. Fernandez-Vina and {Del Theiler}, {C. G.} and Marcos, {Y. C.} and N. Lindel and M. Capucchio and C. Gorodezky and L. Fainboim and P. Stastny",
year = "1995",
language = "English (US)",
volume = "46",
pages = "196--203",
journal = "HLA",
issn = "2059-2302",
publisher = "Wiley-Blackwell",
number = "3 I",

}

TY - JOUR

T1 - Allelic heterogeneity of HLA-B35 subtypes in different populations as assessed by DNA typing

AU - Satz, M. L.

AU - Fernandez-Vina, M.

AU - Del Theiler, C. G.

AU - Marcos, Y. C.

AU - Lindel, N.

AU - Capucchio, M.

AU - Gorodezky, C.

AU - Fainboim, L.

AU - Stastny, P.

PY - 1995

Y1 - 1995

N2 - HLA-B35, a class I antigen differentially associated to several diseases in different ethnic groups, comprises at least eight alleles which differ among them by one to six amino acids. In the present work a rapid DNA typing procedure was used to investigate the distribution of the various HLA-B35 alleles in different populations. The approach is based on a group-specific PCR amplification of a set of closely related HLA-B alleles sharing a Thr in position 45 of the alpha-1 domain. The amplified DNA was then hybridized to a panel of sequence-specific oligonucleotide (SSO) probes designed to recognize the polymorphic residues in previously reported HLA-B35 subtypes. This methodology was successfully tested in 100 individuals of four different populations, previously typed by serology as HLA-B35, and in six reference panel cells of the 10th International Histocompatibility Workshop. HLA-B(*)3501 was the predominant subtype in all populations. B(*)3502, B(*)3503 and, to a lesser extent B(*)3508, were also found. Among Mexican Mestizos, thirteen individuals had patterns of SSO hybridization suggestive of new B35 alleles. The evolutionary considerations on the different B35 alleles and their extended B35,Cw4 haplotypes are discussed.

AB - HLA-B35, a class I antigen differentially associated to several diseases in different ethnic groups, comprises at least eight alleles which differ among them by one to six amino acids. In the present work a rapid DNA typing procedure was used to investigate the distribution of the various HLA-B35 alleles in different populations. The approach is based on a group-specific PCR amplification of a set of closely related HLA-B alleles sharing a Thr in position 45 of the alpha-1 domain. The amplified DNA was then hybridized to a panel of sequence-specific oligonucleotide (SSO) probes designed to recognize the polymorphic residues in previously reported HLA-B35 subtypes. This methodology was successfully tested in 100 individuals of four different populations, previously typed by serology as HLA-B35, and in six reference panel cells of the 10th International Histocompatibility Workshop. HLA-B(*)3501 was the predominant subtype in all populations. B(*)3502, B(*)3503 and, to a lesser extent B(*)3508, were also found. Among Mexican Mestizos, thirteen individuals had patterns of SSO hybridization suggestive of new B35 alleles. The evolutionary considerations on the different B35 alleles and their extended B35,Cw4 haplotypes are discussed.

KW - DNA typing

KW - Group-specific amplification

KW - HLA-B35 allele

KW - PCR

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=0029162737&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029162737&partnerID=8YFLogxK

M3 - Article

C2 - 8525479

AN - SCOPUS:0029162737

VL - 46

SP - 196

EP - 203

JO - HLA

JF - HLA

SN - 2059-2302

IS - 3 I

ER -