Allelic losses at chromosome 3p are seen in human papilloma virus 16 associated transitional cell carcinoma of the cervix

Anirban Maitra, Ignacio I. Wistuba, David Gibbons, Adi F. Gazdar, Jorge Albores-Saavedra

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective. Transitional cell carcinomas (TCCs) of the cervix are rare neoplasms of the female genital tract. Although these tumors display urothelial differentiation, there is controversy regarding their histogenetic relationship to squamous cell carcinomas (SCC) of the cervix versus transitional cell carcinomas of the bladder. Methods. We performed partial allelotyping of five TCCs of the cervix using 23 polymorphic markers located on chromosomes 3p and 9, which demonstrate frequent and early losses in cervical SCC and urothelial TCC, respectively. Multiplex polymerase chain reaction was used on DNA extracted from archival paraffin-embedded tissue using precise microdissection. Additionally, P53 gene mutation analysis was performed using single-strand confirmation polymorphism (SSCP) and the presence of human papilloma virus (HPV) sequences was analyzed using general and specific (types 16 and 18) primers. Results. General HPV sequences were demonstrated in all cases, but the oncogenic strain HPV 16 was present in only three (60%) of the five tumors; no HPV 18 was detected in any sample. Three of five TCCs, all harboring HPV 16 sequences, demonstrated concurrent allelic losses at several 3p loci (specifically 3p12, 3p 14.2 [the FHIT gene locus], 3p21.3, and 3p22-24.2). LOH at a single locus on 9q32-qter was demonstrated in one tumor; no other deletions were seen on chromosome 9. P53 gene mutations in exons 5-8 were absent by SSCP analysis. Conclusions. The infrequent involvement of chromosome 9 in TCCs of the cervix, along with the concurrent presence of 3p LOH and oncogenic HPV 16 in a subset of tumors, suggests a closer histogenetic relationship of this neoplasm to cervical SCCs rather than urothelial TCCs.

Original languageEnglish (US)
Pages (from-to)361-368
Number of pages8
JournalGynecologic Oncology
Volume74
Issue number3
DOIs
StatePublished - Sep 1999

Fingerprint

Papillomaviridae
Transitional Cell Carcinoma
Loss of Heterozygosity
Cervix Uteri
Chromosomes
Chromosomes, Human, Pair 9
p53 Genes
Uterine Cervical Neoplasms
Squamous Cell Carcinoma
Neoplasms
Oncogenic Viruses
Microdissection
Mutation
Multiplex Polymerase Chain Reaction
Paraffin
Exons
Urinary Bladder

Keywords

  • Cervix
  • Chromosome 3
  • Chromosome 9
  • Human papilloma virus
  • P53
  • Transitional cell carcinoma

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

Cite this

Allelic losses at chromosome 3p are seen in human papilloma virus 16 associated transitional cell carcinoma of the cervix. / Maitra, Anirban; Wistuba, Ignacio I.; Gibbons, David; Gazdar, Adi F.; Albores-Saavedra, Jorge.

In: Gynecologic Oncology, Vol. 74, No. 3, 09.1999, p. 361-368.

Research output: Contribution to journalArticle

Maitra, Anirban ; Wistuba, Ignacio I. ; Gibbons, David ; Gazdar, Adi F. ; Albores-Saavedra, Jorge. / Allelic losses at chromosome 3p are seen in human papilloma virus 16 associated transitional cell carcinoma of the cervix. In: Gynecologic Oncology. 1999 ; Vol. 74, No. 3. pp. 361-368.
@article{a7732cbb667143e99f3d903916f79b83,
title = "Allelic losses at chromosome 3p are seen in human papilloma virus 16 associated transitional cell carcinoma of the cervix",
abstract = "Objective. Transitional cell carcinomas (TCCs) of the cervix are rare neoplasms of the female genital tract. Although these tumors display urothelial differentiation, there is controversy regarding their histogenetic relationship to squamous cell carcinomas (SCC) of the cervix versus transitional cell carcinomas of the bladder. Methods. We performed partial allelotyping of five TCCs of the cervix using 23 polymorphic markers located on chromosomes 3p and 9, which demonstrate frequent and early losses in cervical SCC and urothelial TCC, respectively. Multiplex polymerase chain reaction was used on DNA extracted from archival paraffin-embedded tissue using precise microdissection. Additionally, P53 gene mutation analysis was performed using single-strand confirmation polymorphism (SSCP) and the presence of human papilloma virus (HPV) sequences was analyzed using general and specific (types 16 and 18) primers. Results. General HPV sequences were demonstrated in all cases, but the oncogenic strain HPV 16 was present in only three (60{\%}) of the five tumors; no HPV 18 was detected in any sample. Three of five TCCs, all harboring HPV 16 sequences, demonstrated concurrent allelic losses at several 3p loci (specifically 3p12, 3p 14.2 [the FHIT gene locus], 3p21.3, and 3p22-24.2). LOH at a single locus on 9q32-qter was demonstrated in one tumor; no other deletions were seen on chromosome 9. P53 gene mutations in exons 5-8 were absent by SSCP analysis. Conclusions. The infrequent involvement of chromosome 9 in TCCs of the cervix, along with the concurrent presence of 3p LOH and oncogenic HPV 16 in a subset of tumors, suggests a closer histogenetic relationship of this neoplasm to cervical SCCs rather than urothelial TCCs.",
keywords = "Cervix, Chromosome 3, Chromosome 9, Human papilloma virus, P53, Transitional cell carcinoma",
author = "Anirban Maitra and Wistuba, {Ignacio I.} and David Gibbons and Gazdar, {Adi F.} and Jorge Albores-Saavedra",
year = "1999",
month = "9",
doi = "10.1006/gyno.1999.5489",
language = "English (US)",
volume = "74",
pages = "361--368",
journal = "Gynecologic Oncology",
issn = "0090-8258",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Allelic losses at chromosome 3p are seen in human papilloma virus 16 associated transitional cell carcinoma of the cervix

AU - Maitra, Anirban

AU - Wistuba, Ignacio I.

AU - Gibbons, David

AU - Gazdar, Adi F.

AU - Albores-Saavedra, Jorge

PY - 1999/9

Y1 - 1999/9

N2 - Objective. Transitional cell carcinomas (TCCs) of the cervix are rare neoplasms of the female genital tract. Although these tumors display urothelial differentiation, there is controversy regarding their histogenetic relationship to squamous cell carcinomas (SCC) of the cervix versus transitional cell carcinomas of the bladder. Methods. We performed partial allelotyping of five TCCs of the cervix using 23 polymorphic markers located on chromosomes 3p and 9, which demonstrate frequent and early losses in cervical SCC and urothelial TCC, respectively. Multiplex polymerase chain reaction was used on DNA extracted from archival paraffin-embedded tissue using precise microdissection. Additionally, P53 gene mutation analysis was performed using single-strand confirmation polymorphism (SSCP) and the presence of human papilloma virus (HPV) sequences was analyzed using general and specific (types 16 and 18) primers. Results. General HPV sequences were demonstrated in all cases, but the oncogenic strain HPV 16 was present in only three (60%) of the five tumors; no HPV 18 was detected in any sample. Three of five TCCs, all harboring HPV 16 sequences, demonstrated concurrent allelic losses at several 3p loci (specifically 3p12, 3p 14.2 [the FHIT gene locus], 3p21.3, and 3p22-24.2). LOH at a single locus on 9q32-qter was demonstrated in one tumor; no other deletions were seen on chromosome 9. P53 gene mutations in exons 5-8 were absent by SSCP analysis. Conclusions. The infrequent involvement of chromosome 9 in TCCs of the cervix, along with the concurrent presence of 3p LOH and oncogenic HPV 16 in a subset of tumors, suggests a closer histogenetic relationship of this neoplasm to cervical SCCs rather than urothelial TCCs.

AB - Objective. Transitional cell carcinomas (TCCs) of the cervix are rare neoplasms of the female genital tract. Although these tumors display urothelial differentiation, there is controversy regarding their histogenetic relationship to squamous cell carcinomas (SCC) of the cervix versus transitional cell carcinomas of the bladder. Methods. We performed partial allelotyping of five TCCs of the cervix using 23 polymorphic markers located on chromosomes 3p and 9, which demonstrate frequent and early losses in cervical SCC and urothelial TCC, respectively. Multiplex polymerase chain reaction was used on DNA extracted from archival paraffin-embedded tissue using precise microdissection. Additionally, P53 gene mutation analysis was performed using single-strand confirmation polymorphism (SSCP) and the presence of human papilloma virus (HPV) sequences was analyzed using general and specific (types 16 and 18) primers. Results. General HPV sequences were demonstrated in all cases, but the oncogenic strain HPV 16 was present in only three (60%) of the five tumors; no HPV 18 was detected in any sample. Three of five TCCs, all harboring HPV 16 sequences, demonstrated concurrent allelic losses at several 3p loci (specifically 3p12, 3p 14.2 [the FHIT gene locus], 3p21.3, and 3p22-24.2). LOH at a single locus on 9q32-qter was demonstrated in one tumor; no other deletions were seen on chromosome 9. P53 gene mutations in exons 5-8 were absent by SSCP analysis. Conclusions. The infrequent involvement of chromosome 9 in TCCs of the cervix, along with the concurrent presence of 3p LOH and oncogenic HPV 16 in a subset of tumors, suggests a closer histogenetic relationship of this neoplasm to cervical SCCs rather than urothelial TCCs.

KW - Cervix

KW - Chromosome 3

KW - Chromosome 9

KW - Human papilloma virus

KW - P53

KW - Transitional cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=0032843151&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032843151&partnerID=8YFLogxK

U2 - 10.1006/gyno.1999.5489

DO - 10.1006/gyno.1999.5489

M3 - Article

C2 - 10479494

AN - SCOPUS:0032843151

VL - 74

SP - 361

EP - 368

JO - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 3

ER -