Alloantibodies against donor epidermis and early kidney transplant rejection

J. R. Moraes, M. E. Moraes, Y. Luo, P. Stastny

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have previously observed that transplant recipient sera with endothelial antibodies are bound to epidermal cells, as shown by immunofluorescence on sections of skin. It was also reported that early kidney failures that occurred despite negative T cell crossmatches were associated with, and could have been predicted by, a crossmatch with donor skin. Ninety patients undergoing kidney transplantation have now been evaluated using this technique. A few other patients were excluded from the analysis because of the presence of autoantibodies staining autologous skin. In 12 the crossmatch with donor skin was positive, and 9 of them had severe rejection within the first 10 days after transplantation. The three patients with a positive skin crossmatch and a benign course had not been tested with autologous skin and therefore autoantibodies could not be excluded. Only 7 of the 78 patients with negative skin crossmatches had early rejection. The correlation between skin crossmatch and early rejection was statistically highly significant (P<0.0001). Studies by flow cytometry have shown that these antigens are found on the surface of epidermal and endothelial cells, and are modulated by gamma interferon. When tested against a panel of skin donors, skin alloantibodies gave different patterns of positive and negative reactions, suggesting polymorphism.

Original languageEnglish (US)
Pages (from-to)370-373
Number of pages4
JournalTransplantation
Volume51
Issue number2
StatePublished - 1991

Fingerprint

Isoantibodies
Graft Rejection
Epidermis
Tissue Donors
Kidney
Skin
Autoantibodies
Kidney Transplantation
Interferon-gamma
Renal Insufficiency
Fluorescent Antibody Technique
Flow Cytometry
Endothelial Cells
Transplantation
Staining and Labeling
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Moraes, J. R., Moraes, M. E., Luo, Y., & Stastny, P. (1991). Alloantibodies against donor epidermis and early kidney transplant rejection. Transplantation, 51(2), 370-373.

Alloantibodies against donor epidermis and early kidney transplant rejection. / Moraes, J. R.; Moraes, M. E.; Luo, Y.; Stastny, P.

In: Transplantation, Vol. 51, No. 2, 1991, p. 370-373.

Research output: Contribution to journalArticle

Moraes, JR, Moraes, ME, Luo, Y & Stastny, P 1991, 'Alloantibodies against donor epidermis and early kidney transplant rejection', Transplantation, vol. 51, no. 2, pp. 370-373.
Moraes JR, Moraes ME, Luo Y, Stastny P. Alloantibodies against donor epidermis and early kidney transplant rejection. Transplantation. 1991;51(2):370-373.
Moraes, J. R. ; Moraes, M. E. ; Luo, Y. ; Stastny, P. / Alloantibodies against donor epidermis and early kidney transplant rejection. In: Transplantation. 1991 ; Vol. 51, No. 2. pp. 370-373.
@article{1c332b49367a4822b4f995a55f70975e,
title = "Alloantibodies against donor epidermis and early kidney transplant rejection",
abstract = "We have previously observed that transplant recipient sera with endothelial antibodies are bound to epidermal cells, as shown by immunofluorescence on sections of skin. It was also reported that early kidney failures that occurred despite negative T cell crossmatches were associated with, and could have been predicted by, a crossmatch with donor skin. Ninety patients undergoing kidney transplantation have now been evaluated using this technique. A few other patients were excluded from the analysis because of the presence of autoantibodies staining autologous skin. In 12 the crossmatch with donor skin was positive, and 9 of them had severe rejection within the first 10 days after transplantation. The three patients with a positive skin crossmatch and a benign course had not been tested with autologous skin and therefore autoantibodies could not be excluded. Only 7 of the 78 patients with negative skin crossmatches had early rejection. The correlation between skin crossmatch and early rejection was statistically highly significant (P<0.0001). Studies by flow cytometry have shown that these antigens are found on the surface of epidermal and endothelial cells, and are modulated by gamma interferon. When tested against a panel of skin donors, skin alloantibodies gave different patterns of positive and negative reactions, suggesting polymorphism.",
author = "Moraes, {J. R.} and Moraes, {M. E.} and Y. Luo and P. Stastny",
year = "1991",
language = "English (US)",
volume = "51",
pages = "370--373",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Alloantibodies against donor epidermis and early kidney transplant rejection

AU - Moraes, J. R.

AU - Moraes, M. E.

AU - Luo, Y.

AU - Stastny, P.

PY - 1991

Y1 - 1991

N2 - We have previously observed that transplant recipient sera with endothelial antibodies are bound to epidermal cells, as shown by immunofluorescence on sections of skin. It was also reported that early kidney failures that occurred despite negative T cell crossmatches were associated with, and could have been predicted by, a crossmatch with donor skin. Ninety patients undergoing kidney transplantation have now been evaluated using this technique. A few other patients were excluded from the analysis because of the presence of autoantibodies staining autologous skin. In 12 the crossmatch with donor skin was positive, and 9 of them had severe rejection within the first 10 days after transplantation. The three patients with a positive skin crossmatch and a benign course had not been tested with autologous skin and therefore autoantibodies could not be excluded. Only 7 of the 78 patients with negative skin crossmatches had early rejection. The correlation between skin crossmatch and early rejection was statistically highly significant (P<0.0001). Studies by flow cytometry have shown that these antigens are found on the surface of epidermal and endothelial cells, and are modulated by gamma interferon. When tested against a panel of skin donors, skin alloantibodies gave different patterns of positive and negative reactions, suggesting polymorphism.

AB - We have previously observed that transplant recipient sera with endothelial antibodies are bound to epidermal cells, as shown by immunofluorescence on sections of skin. It was also reported that early kidney failures that occurred despite negative T cell crossmatches were associated with, and could have been predicted by, a crossmatch with donor skin. Ninety patients undergoing kidney transplantation have now been evaluated using this technique. A few other patients were excluded from the analysis because of the presence of autoantibodies staining autologous skin. In 12 the crossmatch with donor skin was positive, and 9 of them had severe rejection within the first 10 days after transplantation. The three patients with a positive skin crossmatch and a benign course had not been tested with autologous skin and therefore autoantibodies could not be excluded. Only 7 of the 78 patients with negative skin crossmatches had early rejection. The correlation between skin crossmatch and early rejection was statistically highly significant (P<0.0001). Studies by flow cytometry have shown that these antigens are found on the surface of epidermal and endothelial cells, and are modulated by gamma interferon. When tested against a panel of skin donors, skin alloantibodies gave different patterns of positive and negative reactions, suggesting polymorphism.

UR - http://www.scopus.com/inward/record.url?scp=0026084943&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026084943&partnerID=8YFLogxK

M3 - Article

C2 - 1994530

AN - SCOPUS:0026084943

VL - 51

SP - 370

EP - 373

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 2

ER -