Allogeneic lymphocyte stimulation by human spleen: Identification of cells that are very effective for presenting class II HLA antigens

C. A. Pettaway, E. J. Ball, P. Stastny

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Human spleen cells were fractionated by percoli den-sity gradient centrifugation and by sorting in the FACS with mixtures of fluorescent antibodies against T cells, B cells, monocytes, and Sig-bearing cells. Cells responsible for powerful MLR stimulation were class II HLA antigen-positive and were concentrated in preparations depleted of all the markers listed above. These cells represented 1—2% of the initial spleen cells. They were remarkably more active than other HLA class II anti-gen-positive cells. The procedure described allows rapid enrichment for the responsible cells. It should be useful for further characterization of these cells and for performing studies on their function.

Original languageEnglish (US)
Pages (from-to)874-878
Number of pages5
JournalTransplantation
Volume46
Issue number6
DOIs
StatePublished - Dec 1988

ASJC Scopus subject areas

  • Transplantation

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