Alpha particle mutagenesis of human lymphoblastoid cell lines

S. A. Amundson, D. J. Chen, R. T. Okinaka

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Despite being derived from the same donor, the human lymphoblastoid cell lines WTK1 and TK6 have markedly different responses to low LET radiation. We originally observed that WTK1 was more resistant to the cytotoxic effects of X-irradiation, but significantly more sensitive to mutation induction at both the TK and HPRT loci. In an effort to better understand these properties, we have examined the effects of α-particles on these cells. Relative to TK6, WTK1 has enhanced survival and mutation after both X-ray and α-particle exposure. While the HPRT locus was significantly more mutable in WTK1 as a function of α-particle versus X-ray dose, the TK locus was only slightly more sensitive to a-particle mutagenesis. In addition, the slowly growing TK mutants that constitute the majority of X-ray-induced TK mutants of TK6 were recovered in lower proportions following α-particle exposures. This is consistent with the further finding that in both cell lines, loss of heterozygosity occurred in a smaller fraction of α-induced TK mutants than X-ray-induced mutants. These results are consistent with our previous model suggesting that WTK1 has an error-prone repair pathway that is either missing or deficient in TK6, and further suggest that this pathway may be involved in the processing of α-particle-induced damage.

Original languageEnglish (US)
Pages (from-to)219-226
Number of pages8
JournalInternational Journal of Radiation Biology
Volume70
Issue number2
DOIs
StatePublished - 1996

Fingerprint

Alpha Particles
mutagenesis
Mutagenesis
Alpha particles
human cell lines
cultured cells
alpha particles
Cells
X-Rays
Hypoxanthine Phosphoribosyltransferase
Cell Line
X rays
X-radiation
loci
mutants
mutations
Linear Energy Transfer
Mutation
Loss of Heterozygosity
x rays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Alpha particle mutagenesis of human lymphoblastoid cell lines. / Amundson, S. A.; Chen, D. J.; Okinaka, R. T.

In: International Journal of Radiation Biology, Vol. 70, No. 2, 1996, p. 219-226.

Research output: Contribution to journalArticle

Amundson, S. A. ; Chen, D. J. ; Okinaka, R. T. / Alpha particle mutagenesis of human lymphoblastoid cell lines. In: International Journal of Radiation Biology. 1996 ; Vol. 70, No. 2. pp. 219-226.
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