Alpha2 macroglobulin, a PSA binding protein, is expressed in human prostate stroma

Victor K. Lin, Shih Ya Wang, Nicholas C. Boetticher, Dolores V. Vazquez, Hossein Saboorian, John D. McConnell, Claus Roehrborn

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

BACKGROUND. Benign prostatic hyperplasia (BPH) is characterized as a stromal process. The stroma smooth muscle (SM) may alter its phenotype during the progression of BPH. We have identified gene transcripts that may be differentially expressed in BPH using a differential display method. Among the fragments isolated, α2 macroglobulin (α2-M) is one of the most interesting. α2-M is a binding protein of a variety of proteinases, including prostatic specific antigen (PSA). It also plays roles in molecular trapping and targeting. In this study, we characterized α2-M expression in the human prostate. METHODS. Differential display was used to identify and isolate the differentially expressed transcripts between normal prostate and BPH tissues. RT-PCR, Western blot, in situ hybridization, and immunohistochemistry were utilized to confirm and characterize α2-M expression in the prostate. RESULTS. Real-time RT-PCR results revealed that a 3.2-fold increase in α2-M mRNA expression is observed in BPH compared with normal prostate tissue. A 1.9-fold increase at protein level was also observed. In situ hybridization and immunohistochemistry showed that α2-M expression is primarily localized to the stromal compartment. Cultured primary stroma cells maintained α2-M expression, while prostate epithelial cells had a significantly lower level of α2-M expression. Furthermore, stromal cells in culture produce and secrete α2-M in the medium. CONCLUSIONS. We identified α2-M expression in the human prostate. An increased α2-M expression appears to be associated with BPH. Considering the unique features of its protein binding and targeting properties, α2-M expressed in the prostate may play an important role in regulating benign and malignant prostatic growth.

Original languageEnglish (US)
Pages (from-to)299-308
Number of pages10
JournalProstate
Volume63
Issue number3
DOIs
StatePublished - May 15 2005

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alpha-Macroglobulins
Prostatic Hyperplasia
Prostate
Carrier Proteins
Antigens
In Situ Hybridization
Immunohistochemistry
Macroglobulins
Protein Transport
Stromal Cells
Protein Binding
Smooth Muscle
Real-Time Polymerase Chain Reaction
Peptide Hydrolases
Cell Culture Techniques
Western Blotting
Epithelial Cells
Phenotype
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Alpha macroglobulin
  • Benign prostatic hyperplasia
  • Differential display
  • RT-PCR

ASJC Scopus subject areas

  • Urology

Cite this

Lin, V. K., Wang, S. Y., Boetticher, N. C., Vazquez, D. V., Saboorian, H., McConnell, J. D., & Roehrborn, C. (2005). Alpha2 macroglobulin, a PSA binding protein, is expressed in human prostate stroma. Prostate, 63(3), 299-308. https://doi.org/10.1002/pros.20183

Alpha2 macroglobulin, a PSA binding protein, is expressed in human prostate stroma. / Lin, Victor K.; Wang, Shih Ya; Boetticher, Nicholas C.; Vazquez, Dolores V.; Saboorian, Hossein; McConnell, John D.; Roehrborn, Claus.

In: Prostate, Vol. 63, No. 3, 15.05.2005, p. 299-308.

Research output: Contribution to journalArticle

Lin, VK, Wang, SY, Boetticher, NC, Vazquez, DV, Saboorian, H, McConnell, JD & Roehrborn, C 2005, 'Alpha2 macroglobulin, a PSA binding protein, is expressed in human prostate stroma', Prostate, vol. 63, no. 3, pp. 299-308. https://doi.org/10.1002/pros.20183
Lin VK, Wang SY, Boetticher NC, Vazquez DV, Saboorian H, McConnell JD et al. Alpha2 macroglobulin, a PSA binding protein, is expressed in human prostate stroma. Prostate. 2005 May 15;63(3):299-308. https://doi.org/10.1002/pros.20183
Lin, Victor K. ; Wang, Shih Ya ; Boetticher, Nicholas C. ; Vazquez, Dolores V. ; Saboorian, Hossein ; McConnell, John D. ; Roehrborn, Claus. / Alpha2 macroglobulin, a PSA binding protein, is expressed in human prostate stroma. In: Prostate. 2005 ; Vol. 63, No. 3. pp. 299-308.
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abstract = "BACKGROUND. Benign prostatic hyperplasia (BPH) is characterized as a stromal process. The stroma smooth muscle (SM) may alter its phenotype during the progression of BPH. We have identified gene transcripts that may be differentially expressed in BPH using a differential display method. Among the fragments isolated, α2 macroglobulin (α2-M) is one of the most interesting. α2-M is a binding protein of a variety of proteinases, including prostatic specific antigen (PSA). It also plays roles in molecular trapping and targeting. In this study, we characterized α2-M expression in the human prostate. METHODS. Differential display was used to identify and isolate the differentially expressed transcripts between normal prostate and BPH tissues. RT-PCR, Western blot, in situ hybridization, and immunohistochemistry were utilized to confirm and characterize α2-M expression in the prostate. RESULTS. Real-time RT-PCR results revealed that a 3.2-fold increase in α2-M mRNA expression is observed in BPH compared with normal prostate tissue. A 1.9-fold increase at protein level was also observed. In situ hybridization and immunohistochemistry showed that α2-M expression is primarily localized to the stromal compartment. Cultured primary stroma cells maintained α2-M expression, while prostate epithelial cells had a significantly lower level of α2-M expression. Furthermore, stromal cells in culture produce and secrete α2-M in the medium. CONCLUSIONS. We identified α2-M expression in the human prostate. An increased α2-M expression appears to be associated with BPH. Considering the unique features of its protein binding and targeting properties, α2-M expressed in the prostate may play an important role in regulating benign and malignant prostatic growth.",
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AU - Lin, Victor K.

AU - Wang, Shih Ya

AU - Boetticher, Nicholas C.

AU - Vazquez, Dolores V.

AU - Saboorian, Hossein

AU - McConnell, John D.

AU - Roehrborn, Claus

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N2 - BACKGROUND. Benign prostatic hyperplasia (BPH) is characterized as a stromal process. The stroma smooth muscle (SM) may alter its phenotype during the progression of BPH. We have identified gene transcripts that may be differentially expressed in BPH using a differential display method. Among the fragments isolated, α2 macroglobulin (α2-M) is one of the most interesting. α2-M is a binding protein of a variety of proteinases, including prostatic specific antigen (PSA). It also plays roles in molecular trapping and targeting. In this study, we characterized α2-M expression in the human prostate. METHODS. Differential display was used to identify and isolate the differentially expressed transcripts between normal prostate and BPH tissues. RT-PCR, Western blot, in situ hybridization, and immunohistochemistry were utilized to confirm and characterize α2-M expression in the prostate. RESULTS. Real-time RT-PCR results revealed that a 3.2-fold increase in α2-M mRNA expression is observed in BPH compared with normal prostate tissue. A 1.9-fold increase at protein level was also observed. In situ hybridization and immunohistochemistry showed that α2-M expression is primarily localized to the stromal compartment. Cultured primary stroma cells maintained α2-M expression, while prostate epithelial cells had a significantly lower level of α2-M expression. Furthermore, stromal cells in culture produce and secrete α2-M in the medium. CONCLUSIONS. We identified α2-M expression in the human prostate. An increased α2-M expression appears to be associated with BPH. Considering the unique features of its protein binding and targeting properties, α2-M expressed in the prostate may play an important role in regulating benign and malignant prostatic growth.

AB - BACKGROUND. Benign prostatic hyperplasia (BPH) is characterized as a stromal process. The stroma smooth muscle (SM) may alter its phenotype during the progression of BPH. We have identified gene transcripts that may be differentially expressed in BPH using a differential display method. Among the fragments isolated, α2 macroglobulin (α2-M) is one of the most interesting. α2-M is a binding protein of a variety of proteinases, including prostatic specific antigen (PSA). It also plays roles in molecular trapping and targeting. In this study, we characterized α2-M expression in the human prostate. METHODS. Differential display was used to identify and isolate the differentially expressed transcripts between normal prostate and BPH tissues. RT-PCR, Western blot, in situ hybridization, and immunohistochemistry were utilized to confirm and characterize α2-M expression in the prostate. RESULTS. Real-time RT-PCR results revealed that a 3.2-fold increase in α2-M mRNA expression is observed in BPH compared with normal prostate tissue. A 1.9-fold increase at protein level was also observed. In situ hybridization and immunohistochemistry showed that α2-M expression is primarily localized to the stromal compartment. Cultured primary stroma cells maintained α2-M expression, while prostate epithelial cells had a significantly lower level of α2-M expression. Furthermore, stromal cells in culture produce and secrete α2-M in the medium. CONCLUSIONS. We identified α2-M expression in the human prostate. An increased α2-M expression appears to be associated with BPH. Considering the unique features of its protein binding and targeting properties, α2-M expressed in the prostate may play an important role in regulating benign and malignant prostatic growth.

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