Alteration in N-glycomics during mouse aging: A role for FUT8

Valerie Vanhooren, Sylviane Dewaele, Makoto Kuro-o, Naoyuki Taniguchi, Laurent Dollé, Leo A. van Grunsven, Evgenia Makrantonaki, Christos C. Zouboulis, Cuiying C. Chen, Claude Libert

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We recently reported that N-glycosylation changes during human aging. To further investigate the molecular basis determining these alterations, the aging process in mice was studied. N-glycan profiling of mouse serum glycoproteins in different age groups of healthy C57BL/6 mice showed substantial age-related changes in three major N-glycan structures: under-galactosylated biantennary (NGA2F), biantennary (NA2), and core α-1,6-fucosylated -β-galactosylated biantennary structures (NA2F). Mice defective in klotho gene expression (kl/kl), which have a shortened lifespan, displayed a similar but accelerated trend. Interestingly, the opposite trend was observed in slow-aging Snell Dwarf mice (dw/dw) and in mice fed a calorically restricted diet. We also discovered that increased expression and activity of α-1,6-fucosyltransferase (FUT8) in the liver are strongly linked to the age-related changes in glycosylation and that this increased FUT8 and fucosylation influence IGF-1 signaling. These data demonstrate that the glycosylation machinery in liver cells is significantly affected during aging and that age-related increased FUT8 activity could influence the aging process by altering the sensitivity of the IGF-1R signaling pathway.

Original languageEnglish (US)
Pages (from-to)1056-1066
Number of pages11
JournalAging Cell
Volume10
Issue number6
DOIs
StatePublished - Dec 1 2011

Keywords

  • Aging
  • Caloric restriction
  • Glycosylation
  • Klotho mouse
  • N-glycan
  • Snell Dwarf

ASJC Scopus subject areas

  • Aging
  • Cell Biology

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    Vanhooren, V., Dewaele, S., Kuro-o, M., Taniguchi, N., Dollé, L., van Grunsven, L. A., Makrantonaki, E., Zouboulis, C. C., Chen, C. C., & Libert, C. (2011). Alteration in N-glycomics during mouse aging: A role for FUT8. Aging Cell, 10(6), 1056-1066. https://doi.org/10.1111/j.1474-9726.2011.00749.x