Alteration of Rb binding to HPV 18 E7 modified by transglutaminase 2 with different type of polyamines

Ju Hong Jeon, Sung Yup Cho, Chai Wan Kim, Dong Myung Shin, Gi Yong Jang, Hye Jin Lee, Heun Soo Kang, Sang Chul Park, In Gyu Kim

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

High-risk human papillomavirus (HPV) E7 is a major oncoprotein that plays a crucial role in the development of cervical cancer. A previous study showed that transglutaminase (TGase) 2 catalyzes the incorporation of polyamines into HPV 18 E7 protein, and thereby diminishes its ability to bind Rb. Therefore, TGase 2 activity may be implicated in a suppressive function of host against HPV-induced carcinogenesis. To better understand the nature of polyanimation of HPV 18 E7, we investigated the Rb binding of E7 polyaminated in vitro with different type of polyamines. The incorporation of spermine diminished the Rb binding of E7 more profoundly compared with that of spermidine, suggesting that either the additional positive charge or a steric effect or both may have altered the chemical or structural properties of the protein. In addition, the treatment of either spermidine or spermine in cultured cell system reduced the ability of E7 to inactivate Rb with a TGase activity-dependent manner. Spermine was more effective in inhibiting E7 activity than spermidine. These results may provide the basis for future investigation aiming at delineating the significance of polyamine metabolism on HPV E7 functions.

Original languageEnglish (US)
Pages (from-to)1540-1548
Number of pages9
JournalFrontiers in Bioscience
Volume11
Issue number2 P.1199-1590
StatePublished - Feb 23 2006

Keywords

  • Human Papillomavirus E7
  • Polyamination; Spermine
  • Spermidine
  • Transglutaminase 2

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Jeon, J. H., Cho, S. Y., Kim, C. W., Shin, D. M., Jang, G. Y., Lee, H. J., Kang, H. S., Park, S. C., & Kim, I. G. (2006). Alteration of Rb binding to HPV 18 E7 modified by transglutaminase 2 with different type of polyamines. Frontiers in Bioscience, 11(2 P.1199-1590), 1540-1548.