Alteration of the CD34+ Tf-1β cell line profile in response to long-term exposure to IL-15

Nancy L. Farner, Jacek Gan, Jill L O De Jong, Thomas P. Leary, Timothy S. Fenske, Patrick Buckley, Sabrina Dunlap, Paul M. Sondel

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Interleukin 15 (IL-15) is a cytokine with many functional characteristics that are similar to IL-2. Most of the functional activities that IL-2 and IL-15 support have been evaluated in short-term assays. It was our intention, then, to determine the long-term effects of IL-15 in comparison to IL-2. These studies were performed using the growth factor-dependent myelomonocytic cell line, Tf-1, which has been well characterized with regard to morphology, CD marker expression, responses to certain growth factors and cytokines (GM-CSF, IL-4, erythropoietin), and can differentiate through the myeloid and erythroid lineages. In order to study IL-2 and IL-15 responses, Tf-1 cells were retrovirally infected with the IL-2Rβ chain gene as a means to confer IL-2 responsiveness to this cell type. The results of this study demonstrate that retroviral infection of Tf-1 successfully generated a stable 1L-2 responsive cell line, Tf-1β, without interfering with the original characteristics of the Tf-1 cell. Tf-1β cells respond functionally to both IL-2 and IL-15. When Tf-1β cells are grown for 8 weeks in IL-2 (Tf-1β2), rather than GM-CSF, the original morphology, CD marker expression, esterase activity and proliferative response is unaltered in comparison to that of the original Tf-1β line maintained in GM-CSF. However, long-term growth of Tf-1β in IL-15 (Tf-1β15) results in morphological alterations, downregulation of CD33, CD38, and HLA-DR, and a decreased response to IL-15 in comparison to Tf-1β2. These studies support the concept that retroviral infection, even when it confers new functions upon a cell, does not necessarily alter all other functions, as assessed by evaluation of its phenotypic profile. Furthermore, the production of the Tf-1β2 and Tf-1β15 sublines demonstrates that IL-2 and IL-15 can support long-term cell growth. However, this long-term growth in IL-15 leads to subtle alterations in the cell profile that are not seen with IL-2, suggesting that distinctions in IL-2 and IL-15 function do exist. Further study of the Tf-1β15 cell line will be useful to clarify these functional distinctions between IL-2 and IL-15.

Original languageEnglish (US)
Pages (from-to)316-327
Number of pages12
JournalCytokine
Volume9
Issue number5
DOIs
StatePublished - May 1997

Fingerprint

Interleukin-15
Interleukin-2
Cells
Cell Line
Granulocyte-Macrophage Colony-Stimulating Factor
Intercellular Signaling Peptides and Proteins
Cytokines
Cell growth
HLA-DR Antigens
Esterases
Erythropoietin
Growth
Interleukin-4
Assays
Genes

Keywords

  • CD34
  • IL-15
  • Retrovirus
  • TF-1β cell line

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Farner, N. L., Gan, J., De Jong, J. L. O., Leary, T. P., Fenske, T. S., Buckley, P., ... Sondel, P. M. (1997). Alteration of the CD34+ Tf-1β cell line profile in response to long-term exposure to IL-15. Cytokine, 9(5), 316-327. https://doi.org/10.1006/cyto.1996.0171

Alteration of the CD34+ Tf-1β cell line profile in response to long-term exposure to IL-15. / Farner, Nancy L.; Gan, Jacek; De Jong, Jill L O; Leary, Thomas P.; Fenske, Timothy S.; Buckley, Patrick; Dunlap, Sabrina; Sondel, Paul M.

In: Cytokine, Vol. 9, No. 5, 05.1997, p. 316-327.

Research output: Contribution to journalArticle

Farner, NL, Gan, J, De Jong, JLO, Leary, TP, Fenske, TS, Buckley, P, Dunlap, S & Sondel, PM 1997, 'Alteration of the CD34+ Tf-1β cell line profile in response to long-term exposure to IL-15', Cytokine, vol. 9, no. 5, pp. 316-327. https://doi.org/10.1006/cyto.1996.0171
Farner, Nancy L. ; Gan, Jacek ; De Jong, Jill L O ; Leary, Thomas P. ; Fenske, Timothy S. ; Buckley, Patrick ; Dunlap, Sabrina ; Sondel, Paul M. / Alteration of the CD34+ Tf-1β cell line profile in response to long-term exposure to IL-15. In: Cytokine. 1997 ; Vol. 9, No. 5. pp. 316-327.
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