Alterations in cerebellar germinal cell division induced by graft-versus-host disease

W. S T Griffin, E. N. Crom, J. R. Head

Research output: Contribution to journalArticle

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Abstract

A systemic immunological syndrome, graft-versus-host disease (GVHD), which does not cause inflammation or cell death in the cerebellum, is shown to retard granule cell production by decreasing the rate of DNA synthesis (S phase) and prolonging mitosis (M), at metaphase. the rate of cell production in diseased animals at postnatal day 14, quantitated by analysis of the rate of labeling of DNA with 3H-thymidine (3H-Tdr), revealed decreased ability to synthesize new DNA. The number of cells taking up 3H-Tdr label per mm2, as detected by autoradiography, was similar in 14-day-old GVHD and control tissue as was the area of the germinal matrix zone and the number of mitotically active germinal cells per mm2 in sagittal sections near the midline. However, because the total volume of the cerebellum was less, the total Mauthner of mitotically active cells in the whole cerebellum of 11-, 14-, and 17-day-old diseased animals was less than in littermate controls. Furthermore, DNA synthesis per mitotically active germinal cell was less in diseased animals at each age examined. The mitotic index was unaffected until late in the disease (day 17), suggesting that a prolongation of the cell cycle was responsible for this GVHD-induced decrease in DNA synthesis. Consistent with a prolongation of the cell cycle was the finding that the mitotic figures in 14-day-old GVHD cerebella were mostly metaphase figures, whereas those in control cerebella were, as predicted, mostly prophase. Prolongation of the cerebellar cell cycle in 11- and 14-day-old diseased animals may explain the dramatic decrease in the mitotic index, the thickness of the germinal matrix zone, and the number of germinal cells at postnatal day 17.

Original languageEnglish (US)
Pages (from-to)91-101
Number of pages11
JournalJournal of Comparative Neurology
Volume203
Issue number1
StatePublished - 1981

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Graft vs Host Disease
Animal Diseases
Cell Division
Cerebellum
DNA
Cell Cycle
Mitotic Index
Metaphase
Blastodisc
Cell Count
Prophase
Autoradiography
S Phase
Mitosis
Thymidine
Cause of Death
Cell Death
Inflammation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Alterations in cerebellar germinal cell division induced by graft-versus-host disease. / Griffin, W. S T; Crom, E. N.; Head, J. R.

In: Journal of Comparative Neurology, Vol. 203, No. 1, 1981, p. 91-101.

Research output: Contribution to journalArticle

Griffin, W. S T ; Crom, E. N. ; Head, J. R. / Alterations in cerebellar germinal cell division induced by graft-versus-host disease. In: Journal of Comparative Neurology. 1981 ; Vol. 203, No. 1. pp. 91-101.
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