TY - JOUR
T1 - Alterations in erythrocyte glutathione metabolism associated with cervical dysplasias and carcinoma in situ
AU - Basu, Jayasri
AU - Duttagupta, Chandralekha
AU - Vermund, Sten H.
AU - Ahn, Chul
AU - Palan, Prabhudas R.
AU - Romney, Seymour L.
N1 - Funding Information:
This work was supported in part by the Friends of Gynecologic Cancer Research of the Albert Einstein College of Medicine (Bronx, NY) and a grant-in-aid from Hoffmann-LaRoche (Nutley. NJ). We thank Ms. Annette Dwyer for her assistance in patient recruitment and Ms. Ana Martinez for her assistance in retrieving clinical reports.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1993
Y1 - 1993
N2 - The study was designed to test the hypothesis whether cervical dysplasias of the more severe grades are associated with elevated erythrocyte glutathione levels. Subjects were women who obtained Pap tests and were subsequently found (1) not to have any cervical lesions or (2) to have colposcopically visualized, biopsy-confirmed cervical abnormalities histopathologically diagnosed as mild, moderate, severe dysplasias, or carcinoma in situ (CIS). The erythrocyte levels of reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD), and 6-phosphogluconate dehydrogenase (6PGD) were analyzed from coded peripheral venous blood samples. GSH and GR concentrations increased with increasing severity of dysplasia. Exploratory data analysis and multiple pairwise comparisons suggested comparable levels of the glutathione-related variables between these histopathological pairs' (1) mild and moderate dysplasias or (2) severe dysplasia and CIS. We suggest that the changes in erythrocyte glutathione-related indices in conjunction with histopathological diagnosis may have the potential to distinguish between low-and high-grade cervical dysplastic lesions.
AB - The study was designed to test the hypothesis whether cervical dysplasias of the more severe grades are associated with elevated erythrocyte glutathione levels. Subjects were women who obtained Pap tests and were subsequently found (1) not to have any cervical lesions or (2) to have colposcopically visualized, biopsy-confirmed cervical abnormalities histopathologically diagnosed as mild, moderate, severe dysplasias, or carcinoma in situ (CIS). The erythrocyte levels of reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD), and 6-phosphogluconate dehydrogenase (6PGD) were analyzed from coded peripheral venous blood samples. GSH and GR concentrations increased with increasing severity of dysplasia. Exploratory data analysis and multiple pairwise comparisons suggested comparable levels of the glutathione-related variables between these histopathological pairs' (1) mild and moderate dysplasias or (2) severe dysplasia and CIS. We suggest that the changes in erythrocyte glutathione-related indices in conjunction with histopathological diagnosis may have the potential to distinguish between low-and high-grade cervical dysplastic lesions.
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U2 - 10.3109/07357909309046937
DO - 10.3109/07357909309046937
M3 - Article
C2 - 8221197
AN - SCOPUS:0027370775
SN - 0735-7907
VL - 11
SP - 652
EP - 659
JO - Cancer Investigation
JF - Cancer Investigation
IS - 6
ER -