Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays

Donna M. Gadbois, Harry A. Crissman, Anthony Nastasi, Robb Habbersett, Sha Ke Wang, David Chen, Bruce E. Lehnert

Research output: Contribution to journalArticle

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Abstract

A G1-phase delay after exposure to α particles has not been reported previously, perhaps because immortalized cell lines or cell lines from tumor cells were used in past studies. Therefore, we compared the effects of α particles (0.19 or 0.57 Gy) and approximately equitoxic doses of γ/rays (2 or 4 Gy) on progression of cells through the cell cycle in normal human skin fibroblasts. Cell cycle analyses were performed using flow cytometry by measuring incorporation of bromodeoxyuridine (BrdUrd) in each phase of the cell cycle up to 44 h after irradiation. We observed an α-particle-induced G1-phase delay in human skin fibroblasts even at the lowest dose, 0.19 Gy. At equitoxic doses, more pronounced and persistent G1-phase delays and arrests were observed in γ-irradiated cultures in that increased fractions of the G1-phase cells remained BrdUrd- over the course of the study after γ-ray exposure compared to cells exposed to α particles. In addition, G1- phase cells that became BrdUrd+ after y irradiation re-arrested in G1 phase, whereas BrdUrd+ G1phase cells in α-particle-irradiated cultures continued cycling. In contrast, comparable percentages of cells were delayed in G2 phase after either α-particle or γ irradiation. Both γ and α- particle irradiation caused increases in cellular p53 and p21(Cip1) shortly after the exposures, which suggests that the G1-phase delay that occurs in response to α-particle irradiation is dependent on p53 like the initial G1- phase delay induced by γ rays.

Original languageEnglish (US)
Pages (from-to)414-424
Number of pages11
JournalRadiation Research
Volume146
Issue number4
StatePublished - Oct 1996

Fingerprint

Alpha Particles
Gamma Rays
G1 Phase
progressions
interphase
gamma radiation
alpha particles
cell cycle
rays
Cell Cycle
gamma rays
cycles
Bromodeoxyuridine
irradiation
cells
fibroblasts
Tumor Cell Line
cultured cells
dosage
skin (animal)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Gadbois, D. M., Crissman, H. A., Nastasi, A., Habbersett, R., Wang, S. K., Chen, D., & Lehnert, B. E. (1996). Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays. Radiation Research, 146(4), 414-424.

Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays. / Gadbois, Donna M.; Crissman, Harry A.; Nastasi, Anthony; Habbersett, Robb; Wang, Sha Ke; Chen, David; Lehnert, Bruce E.

In: Radiation Research, Vol. 146, No. 4, 10.1996, p. 414-424.

Research output: Contribution to journalArticle

Gadbois, DM, Crissman, HA, Nastasi, A, Habbersett, R, Wang, SK, Chen, D & Lehnert, BE 1996, 'Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays', Radiation Research, vol. 146, no. 4, pp. 414-424.
Gadbois DM, Crissman HA, Nastasi A, Habbersett R, Wang SK, Chen D et al. Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays. Radiation Research. 1996 Oct;146(4):414-424.
Gadbois, Donna M. ; Crissman, Harry A. ; Nastasi, Anthony ; Habbersett, Robb ; Wang, Sha Ke ; Chen, David ; Lehnert, Bruce E. / Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays. In: Radiation Research. 1996 ; Vol. 146, No. 4. pp. 414-424.
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