Altered apolipoprotein E secretion in cytokine treated human astrocyte cultures

Fred Baskin, George M. Smith, Jennifer A. Fosmire, Roger N. Rosenberg

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Apolipoprotein E (ApoE), postulated to be a major lipid carrier protein in brain, is synthesized and secreted primarily by astrocytes and is involved in brain development and repair. We have analyzed its secretion in primary cultures of older (high passage) slowly dividing and younger (lower passage) rapidly dividing fetal human astrocytes exposed to various inflammatory and anti-inflammatory cytokines, alone and in combination. ApoE secretion was reduced in high passage astrocytes when compared to lower passage astrocytes. A further reduction in ApoE secretion in high passage cells was consistently produced by the combination of cytokines interleukin 1 (IL-1) alpha and beta and interferon (IFN-gamma) cytokines or by the basic fibroblast growth factor (basic-FGF) alone. Epidermal growth factor (EGF) increased ApoE secretion. The combination of these cytokine effects in chronically degenerating brain regions of Alzheimer's disease and other neurodegenerative diseases could reduce the amount of ApoE available for neuronal regeneration. EGF, or agents inducing EGF, could ameliorate these ApoE deficiencies.

Original languageEnglish (US)
Pages (from-to)15-18
Number of pages4
JournalJournal of the Neurological Sciences
Volume148
Issue number1
DOIs
StatePublished - May 1 1997

Keywords

  • Alzheimer's disease (AD)
  • apolipoprotein E (ApoE)
  • astrocyte
  • basic-fibroblast growth factor (basic-FGF)
  • epidermal growth factor (EGF)
  • interferon-gamma (IFN-gamma)
  • interleukin-1 (IL-1)
  • secretion

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Altered apolipoprotein E secretion in cytokine treated human astrocyte cultures'. Together they form a unique fingerprint.

  • Cite this