Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer

Charles H. Hensel, Chih Lin Hsieh, Adi F. Gazdar, Bruce E. Johnson, Alan Y. Sakaguchi, Susan L. Naylor, Wen Hwa Lee, Eva Y H P Lee

Research output: Contribution to journalArticle

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Abstract

Karyotypic and molecular genetic evidence has indicated that deletion or rearrangement of both chromosomes 3 and 13 may be important in the pathology of human small cell lung cancer (SCLC). The retinoblastoma susceptibility gene, RB, on chromosome 13 band q14, has previously been shown to be altered in SCLC [J. W. Harbour et al., Science (Wash. DC), 241: 353-357, 1988; J. Yokota et al., Oncogene, 3: 471-475, 1988]. Our studies of 26 SCLC tumor and normal DNA samples indicate that 6 of 6 patients whose normal cell DNA was heterozygous for an RB restriction fragment length polymorphism have lost one of the two alleles in their tumor DNA. Consistent with other studies, we find 2 of 26 tumors with homozygous deletions within the RB gene. Of 13 SCLC cell lines examined, only 3 expressed greater than trace amounts of RB mRNA. RB protein was detected in 2 of 14 SCLC cell lines examined, unlike the results of Yokota et al. (Oncogene, 3: 471-475, 1988) which showed no RB protein in any of the 9 cell lines they examined. Only unphosphorylated RB protein was detected in SCLC cell line H209, suggesting that the RB protein may be inactivated by a novel mechanism in this cell line. These data suggest that inactivation of the RB gene is a frequent if not universal event in SCLC.

Original languageEnglish (US)
Pages (from-to)3067-3072
Number of pages6
JournalCancer Research
Volume50
Issue number10
StatePublished - May 15 1990

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Retinoblastoma Genes
Small Cell Lung Carcinoma
Cell Line
Chromosomes, Human, Pair 13
Oncogenes
DNA
Proteins
Neoplasms
Chromosomes, Human, Pair 3
Gene Silencing
Restriction Fragment Length Polymorphisms
Molecular Biology
Alleles
Pathology
Messenger RNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hensel, C. H., Hsieh, C. L., Gazdar, A. F., Johnson, B. E., Sakaguchi, A. Y., Naylor, S. L., ... Lee, E. Y. H. P. (1990). Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Research, 50(10), 3067-3072.

Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. / Hensel, Charles H.; Hsieh, Chih Lin; Gazdar, Adi F.; Johnson, Bruce E.; Sakaguchi, Alan Y.; Naylor, Susan L.; Lee, Wen Hwa; Lee, Eva Y H P.

In: Cancer Research, Vol. 50, No. 10, 15.05.1990, p. 3067-3072.

Research output: Contribution to journalArticle

Hensel, CH, Hsieh, CL, Gazdar, AF, Johnson, BE, Sakaguchi, AY, Naylor, SL, Lee, WH & Lee, EYHP 1990, 'Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer', Cancer Research, vol. 50, no. 10, pp. 3067-3072.
Hensel CH, Hsieh CL, Gazdar AF, Johnson BE, Sakaguchi AY, Naylor SL et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Research. 1990 May 15;50(10):3067-3072.
Hensel, Charles H. ; Hsieh, Chih Lin ; Gazdar, Adi F. ; Johnson, Bruce E. ; Sakaguchi, Alan Y. ; Naylor, Susan L. ; Lee, Wen Hwa ; Lee, Eva Y H P. / Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. In: Cancer Research. 1990 ; Vol. 50, No. 10. pp. 3067-3072.
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