Alternative mRNA splicing generates tissue-specific isoforms of 116-kDa polypeptide of vacuolar proton pump

Sheng Bin Peng, Bill P. Crider, Xiao Song Xie, Dennis K. Stone

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The cDNA encoding the 116-kDa polypeptide of the bovine brain vacuolar- type proton translocating ATPases has been cloned and sequenced. One of five clones differed from all others in that it contained an 18-base pair deletion within the coding region, whereas it was identical to the other clones in overlapping coding and noncoding regions, indicating that this heterogeneity arises through an alternative splicing mechanism. By conventional Northern analysis, only one 4.1-kilobase mRNA was identified in bovine brain, heart, kidney, liver, and spleen. However, a polymerase chain reaction-based analysis revealed two species of mRNA with a tissue-specific distribution. Type I, containing the 18-base pair insert, was found in brain, whereas the truncated (Type II) form was found in all tissues examined. Similar tissue distributions of rat mRNA were observed. The deletion site accounting for this variability occurs within a predicted protease sensitivity motif (PEST site), suggesting that differences in the biological half-life of the two 116-kDa isoforms may exist.

Original languageEnglish (US)
Pages (from-to)17262-17266
Number of pages5
JournalJournal of Biological Chemistry
Volume269
Issue number25
StatePublished - Jun 24 1994

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Proton Pumps
Alternative Splicing
Brain
Protein Isoforms
Tissue Distribution
Tissue
Base Pairing
Messenger RNA
Peptides
Clone Cells
Vacuolar Proton-Translocating ATPases
Polymerase chain reaction
Liver
Half-Life
Rats
Peptide Hydrolases
Spleen
Complementary DNA
Kidney
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry

Cite this

Alternative mRNA splicing generates tissue-specific isoforms of 116-kDa polypeptide of vacuolar proton pump. / Peng, Sheng Bin; Crider, Bill P.; Xie, Xiao Song; Stone, Dennis K.

In: Journal of Biological Chemistry, Vol. 269, No. 25, 24.06.1994, p. 17262-17266.

Research output: Contribution to journalArticle

Peng, Sheng Bin ; Crider, Bill P. ; Xie, Xiao Song ; Stone, Dennis K. / Alternative mRNA splicing generates tissue-specific isoforms of 116-kDa polypeptide of vacuolar proton pump. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 25. pp. 17262-17266.
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