Alternative requirements for vestigial, scalloped, and Dmef2 during muscle differentiation in drosophila melanogaster

Hua Deng, Sarah C. Hughes, John B. Bell, Andrew J. Simmonds

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Vertebrate development requires the activity of the myocyte enhancer factor 2 (mef2) gene family for muscle cell specification and subsequent differentiation. Additionally, several muscle-specific functions of MEF2 family proteins require binding additional cofactors including members of the Transcription Enhancing Factor-1 (TEF-1) and Vestigial- like protein families. In Drosophila there is a single mef2 (Dmef2) gene as well single homologues of TEF-1 and vestigial-like, scalloped (sd), and vestigial (vg), respectively. To clarify the role(s) of these factors, we examined the requirements for Vg and Sd during Drosophila muscle specification. We found that both are required for muscle differentiation as loss of sd or vg leads to a reproducible loss of a subset of either cardiac or somatic muscle cells in developing embryos. This muscle requirement for Sd or Vg is cell specific, as ubiquitous overexpression of either or both of these proteins in muscle cells has a deleterious effect on muscle differentiation. Finally, using both in vitro and in vivo binding assays, we determined that Sd, Vg, and Dmef2 can interact directly. Thus, the muscle-specific phenotypes we have associated with Vg or Sd may be a consequence of alternative binding of Vg and/or Sd to Dmef2 forming alternative protein complexes that modify Dmef2 activity.

Original languageEnglish (US)
Pages (from-to)256-269
Number of pages14
JournalMolecular biology of the cell
Volume20
Issue number1
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

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Drosophila melanogaster
MEF2 Transcription Factors
Muscles
Muscle Cells
Drosophila
Transcription Factors
Proteins
Protein Binding
Genes
Vertebrates
Embryonic Structures
Phenotype

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Alternative requirements for vestigial, scalloped, and Dmef2 during muscle differentiation in drosophila melanogaster. / Deng, Hua; Hughes, Sarah C.; Bell, John B.; Simmonds, Andrew J.

In: Molecular biology of the cell, Vol. 20, No. 1, 01.01.2009, p. 256-269.

Research output: Contribution to journalArticle

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