Alternative splicing of class Ib major histocompatibility complex transcripts in vivo leads to the expression of soluble Qa-2 molecules in murine blood

Piotr Tabaczewski, Haval Shirwan, Keith Lewis, Iwona Stroynowski

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Class Ib Qa-2 molecules are expressed in tissue culture cells as ≃40-kDa membrane-bound, glycophosphatidylinositol-linked antigens and as ≃39-kDa soluble polypeptides. Recently, alternative splicing events which delete exon 5 from a portion of Qa-2 transcripts were demonstrated to give rise to truncated secreted Qa-2 molecules in transfected cell lines. To determine whether this mechanism operates in vivo and to find out whether Qa-2 can be detected in soluble form in circulation, murine blood samples were analyzed. Critical to these experiments was preparation of an anti-peptide antiserum against an epitope encoded by a junction of exon 4 and exon 6. We find that supernatants of splenocytes cultured in vitro as well as serum or plasma contain two forms of soluble Qa-2 molecules. One form corresponds to a secreted molecule translated from transcripts from which exon 5 has been deleted; the other is derived from membrane-bound antigens or their precursors. The levels of both soluble forms of Qa-2 are inducible upon stimulation of the immune system, suggesting an immunoregulatory role for these molecules or for the mechanism leading to the reduction of cell- associated Qa-2 antigens in vivo.

Original languageEnglish (US)
Pages (from-to)1883-1887
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number5
StatePublished - Mar 1 1994

Fingerprint

Alternative Splicing
Major Histocompatibility Complex
Exons
Antigens
Peptides
Membranes
Blood Circulation
Immune Sera
Epitopes
Immune System
Cell Culture Techniques
Cell Line
Serum

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

@article{ad21230d7c154fd7aea12f739e96e4a4,
title = "Alternative splicing of class Ib major histocompatibility complex transcripts in vivo leads to the expression of soluble Qa-2 molecules in murine blood",
abstract = "Class Ib Qa-2 molecules are expressed in tissue culture cells as ≃40-kDa membrane-bound, glycophosphatidylinositol-linked antigens and as ≃39-kDa soluble polypeptides. Recently, alternative splicing events which delete exon 5 from a portion of Qa-2 transcripts were demonstrated to give rise to truncated secreted Qa-2 molecules in transfected cell lines. To determine whether this mechanism operates in vivo and to find out whether Qa-2 can be detected in soluble form in circulation, murine blood samples were analyzed. Critical to these experiments was preparation of an anti-peptide antiserum against an epitope encoded by a junction of exon 4 and exon 6. We find that supernatants of splenocytes cultured in vitro as well as serum or plasma contain two forms of soluble Qa-2 molecules. One form corresponds to a secreted molecule translated from transcripts from which exon 5 has been deleted; the other is derived from membrane-bound antigens or their precursors. The levels of both soluble forms of Qa-2 are inducible upon stimulation of the immune system, suggesting an immunoregulatory role for these molecules or for the mechanism leading to the reduction of cell- associated Qa-2 antigens in vivo.",
author = "Piotr Tabaczewski and Haval Shirwan and Keith Lewis and Iwona Stroynowski",
year = "1994",
month = "3",
day = "1",
language = "English (US)",
volume = "91",
pages = "1883--1887",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "5",

}

TY - JOUR

T1 - Alternative splicing of class Ib major histocompatibility complex transcripts in vivo leads to the expression of soluble Qa-2 molecules in murine blood

AU - Tabaczewski, Piotr

AU - Shirwan, Haval

AU - Lewis, Keith

AU - Stroynowski, Iwona

PY - 1994/3/1

Y1 - 1994/3/1

N2 - Class Ib Qa-2 molecules are expressed in tissue culture cells as ≃40-kDa membrane-bound, glycophosphatidylinositol-linked antigens and as ≃39-kDa soluble polypeptides. Recently, alternative splicing events which delete exon 5 from a portion of Qa-2 transcripts were demonstrated to give rise to truncated secreted Qa-2 molecules in transfected cell lines. To determine whether this mechanism operates in vivo and to find out whether Qa-2 can be detected in soluble form in circulation, murine blood samples were analyzed. Critical to these experiments was preparation of an anti-peptide antiserum against an epitope encoded by a junction of exon 4 and exon 6. We find that supernatants of splenocytes cultured in vitro as well as serum or plasma contain two forms of soluble Qa-2 molecules. One form corresponds to a secreted molecule translated from transcripts from which exon 5 has been deleted; the other is derived from membrane-bound antigens or their precursors. The levels of both soluble forms of Qa-2 are inducible upon stimulation of the immune system, suggesting an immunoregulatory role for these molecules or for the mechanism leading to the reduction of cell- associated Qa-2 antigens in vivo.

AB - Class Ib Qa-2 molecules are expressed in tissue culture cells as ≃40-kDa membrane-bound, glycophosphatidylinositol-linked antigens and as ≃39-kDa soluble polypeptides. Recently, alternative splicing events which delete exon 5 from a portion of Qa-2 transcripts were demonstrated to give rise to truncated secreted Qa-2 molecules in transfected cell lines. To determine whether this mechanism operates in vivo and to find out whether Qa-2 can be detected in soluble form in circulation, murine blood samples were analyzed. Critical to these experiments was preparation of an anti-peptide antiserum against an epitope encoded by a junction of exon 4 and exon 6. We find that supernatants of splenocytes cultured in vitro as well as serum or plasma contain two forms of soluble Qa-2 molecules. One form corresponds to a secreted molecule translated from transcripts from which exon 5 has been deleted; the other is derived from membrane-bound antigens or their precursors. The levels of both soluble forms of Qa-2 are inducible upon stimulation of the immune system, suggesting an immunoregulatory role for these molecules or for the mechanism leading to the reduction of cell- associated Qa-2 antigens in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0028343567&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028343567&partnerID=8YFLogxK

M3 - Article

VL - 91

SP - 1883

EP - 1887

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 5

ER -