Amide proton transfer imaging of adult diffuse gliomas: Correlation with histopathological grades

Osamu Togao, Takashi Yoshiura, Jochen Keupp, Akio Hiwatashi, Koji Yamashita, Kazufumi Kikuchi, Yuriko Suzuki, Satoshi O. Suzuki, Toru Iwaki, Nobuhiro Hata, Masahiro Mizoguchi, Koji Yoshimoto, Koji Sagiyama, Masaya Takahashi, Hiroshi Honda

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

BackgroundAmide proton transfer (APT) imaging is a novel molecular MRI technique to detect endogenous mobile proteins and peptides through chemical exchange saturation transfer. We prospectively assessed the usefulness of APT imaging in predicting the histological grade of adult diffuse gliomas.MethodsThirty-six consecutive patients with histopathologically proven diffuse glioma (48.1 ± 14.7 y old, 16 males and 20 females) were included in the study. APT MRI was conducted on a 3T clinical scanner and was obtained with 2 s saturation at 25 saturation frequency offsets ω =-6 to +6 ppm (step 0.5 ppm). δB0 maps were acquired separately for a point-by-point δB0 correction. APT signal intensity (SI) was defined as magnetization transfer asymmetry at 3.5 ppm: magnetization transfer ratio (MTR)asym = (S[-3.5 ppm]-S [+3.5 ppm])/S0. Regions of interest were carefully placed by 2 neuroradiologists in solid parts within brain tumors. The APT SI was compared with World Health Organization grade, Ki-67 labeling index (LI), and cell density.ResultsThe mean APT SI values were 2.1 ± 0.4% in grade II gliomas (n = 8), 3.2 ± 0.9% in grade III gliomas (n = 10), and 4.1 ± 1.0% in grade IV gliomas (n = 18). Significant differences in APT intensity were observed between grades II and III (P <. 05) and grades III and IV (P <. 05), as well as between grades II and IV (P <. 001). There were positive correlations between APT SI and Ki-67 LI (P =. 01, R = 0.43) and between APT SI and cell density (P <. 05, R = 0.38). The gliomas with microscopic necrosis showed higher APT SI than those without necrosis (P <. 001).ConclusionsAPT imaging can predict the histopathological grades of adult diffuse gliomas.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalNeuro-Oncology
Volume16
Issue number3
DOIs
StatePublished - Mar 2014

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Amides
Glioma
Protons
Necrosis
Cell Count
APT
Brain Neoplasms
Peptides

Keywords

  • amide proton transfer (APT) imaging
  • chemical exchange saturation transfer (CEST)
  • glioma.

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Clinical Neurology

Cite this

Togao, O., Yoshiura, T., Keupp, J., Hiwatashi, A., Yamashita, K., Kikuchi, K., ... Honda, H. (2014). Amide proton transfer imaging of adult diffuse gliomas: Correlation with histopathological grades. Neuro-Oncology, 16(3), 441-448. https://doi.org/10.1093/neuonc/not158

Amide proton transfer imaging of adult diffuse gliomas : Correlation with histopathological grades. / Togao, Osamu; Yoshiura, Takashi; Keupp, Jochen; Hiwatashi, Akio; Yamashita, Koji; Kikuchi, Kazufumi; Suzuki, Yuriko; Suzuki, Satoshi O.; Iwaki, Toru; Hata, Nobuhiro; Mizoguchi, Masahiro; Yoshimoto, Koji; Sagiyama, Koji; Takahashi, Masaya; Honda, Hiroshi.

In: Neuro-Oncology, Vol. 16, No. 3, 03.2014, p. 441-448.

Research output: Contribution to journalArticle

Togao, O, Yoshiura, T, Keupp, J, Hiwatashi, A, Yamashita, K, Kikuchi, K, Suzuki, Y, Suzuki, SO, Iwaki, T, Hata, N, Mizoguchi, M, Yoshimoto, K, Sagiyama, K, Takahashi, M & Honda, H 2014, 'Amide proton transfer imaging of adult diffuse gliomas: Correlation with histopathological grades', Neuro-Oncology, vol. 16, no. 3, pp. 441-448. https://doi.org/10.1093/neuonc/not158
Togao O, Yoshiura T, Keupp J, Hiwatashi A, Yamashita K, Kikuchi K et al. Amide proton transfer imaging of adult diffuse gliomas: Correlation with histopathological grades. Neuro-Oncology. 2014 Mar;16(3):441-448. https://doi.org/10.1093/neuonc/not158
Togao, Osamu ; Yoshiura, Takashi ; Keupp, Jochen ; Hiwatashi, Akio ; Yamashita, Koji ; Kikuchi, Kazufumi ; Suzuki, Yuriko ; Suzuki, Satoshi O. ; Iwaki, Toru ; Hata, Nobuhiro ; Mizoguchi, Masahiro ; Yoshimoto, Koji ; Sagiyama, Koji ; Takahashi, Masaya ; Honda, Hiroshi. / Amide proton transfer imaging of adult diffuse gliomas : Correlation with histopathological grades. In: Neuro-Oncology. 2014 ; Vol. 16, No. 3. pp. 441-448.
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abstract = "BackgroundAmide proton transfer (APT) imaging is a novel molecular MRI technique to detect endogenous mobile proteins and peptides through chemical exchange saturation transfer. We prospectively assessed the usefulness of APT imaging in predicting the histological grade of adult diffuse gliomas.MethodsThirty-six consecutive patients with histopathologically proven diffuse glioma (48.1 ± 14.7 y old, 16 males and 20 females) were included in the study. APT MRI was conducted on a 3T clinical scanner and was obtained with 2 s saturation at 25 saturation frequency offsets ω =-6 to +6 ppm (step 0.5 ppm). δB0 maps were acquired separately for a point-by-point δB0 correction. APT signal intensity (SI) was defined as magnetization transfer asymmetry at 3.5 ppm: magnetization transfer ratio (MTR)asym = (S[-3.5 ppm]-S [+3.5 ppm])/S0. Regions of interest were carefully placed by 2 neuroradiologists in solid parts within brain tumors. The APT SI was compared with World Health Organization grade, Ki-67 labeling index (LI), and cell density.ResultsThe mean APT SI values were 2.1 ± 0.4{\%} in grade II gliomas (n = 8), 3.2 ± 0.9{\%} in grade III gliomas (n = 10), and 4.1 ± 1.0{\%} in grade IV gliomas (n = 18). Significant differences in APT intensity were observed between grades II and III (P <. 05) and grades III and IV (P <. 05), as well as between grades II and IV (P <. 001). There were positive correlations between APT SI and Ki-67 LI (P =. 01, R = 0.43) and between APT SI and cell density (P <. 05, R = 0.38). The gliomas with microscopic necrosis showed higher APT SI than those without necrosis (P <. 001).ConclusionsAPT imaging can predict the histopathological grades of adult diffuse gliomas.",
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AU - Yoshiura, Takashi

AU - Keupp, Jochen

AU - Hiwatashi, Akio

AU - Yamashita, Koji

AU - Kikuchi, Kazufumi

AU - Suzuki, Yuriko

AU - Suzuki, Satoshi O.

AU - Iwaki, Toru

AU - Hata, Nobuhiro

AU - Mizoguchi, Masahiro

AU - Yoshimoto, Koji

AU - Sagiyama, Koji

AU - Takahashi, Masaya

AU - Honda, Hiroshi

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N2 - BackgroundAmide proton transfer (APT) imaging is a novel molecular MRI technique to detect endogenous mobile proteins and peptides through chemical exchange saturation transfer. We prospectively assessed the usefulness of APT imaging in predicting the histological grade of adult diffuse gliomas.MethodsThirty-six consecutive patients with histopathologically proven diffuse glioma (48.1 ± 14.7 y old, 16 males and 20 females) were included in the study. APT MRI was conducted on a 3T clinical scanner and was obtained with 2 s saturation at 25 saturation frequency offsets ω =-6 to +6 ppm (step 0.5 ppm). δB0 maps were acquired separately for a point-by-point δB0 correction. APT signal intensity (SI) was defined as magnetization transfer asymmetry at 3.5 ppm: magnetization transfer ratio (MTR)asym = (S[-3.5 ppm]-S [+3.5 ppm])/S0. Regions of interest were carefully placed by 2 neuroradiologists in solid parts within brain tumors. The APT SI was compared with World Health Organization grade, Ki-67 labeling index (LI), and cell density.ResultsThe mean APT SI values were 2.1 ± 0.4% in grade II gliomas (n = 8), 3.2 ± 0.9% in grade III gliomas (n = 10), and 4.1 ± 1.0% in grade IV gliomas (n = 18). Significant differences in APT intensity were observed between grades II and III (P <. 05) and grades III and IV (P <. 05), as well as between grades II and IV (P <. 001). There were positive correlations between APT SI and Ki-67 LI (P =. 01, R = 0.43) and between APT SI and cell density (P <. 05, R = 0.38). The gliomas with microscopic necrosis showed higher APT SI than those without necrosis (P <. 001).ConclusionsAPT imaging can predict the histopathological grades of adult diffuse gliomas.

AB - BackgroundAmide proton transfer (APT) imaging is a novel molecular MRI technique to detect endogenous mobile proteins and peptides through chemical exchange saturation transfer. We prospectively assessed the usefulness of APT imaging in predicting the histological grade of adult diffuse gliomas.MethodsThirty-six consecutive patients with histopathologically proven diffuse glioma (48.1 ± 14.7 y old, 16 males and 20 females) were included in the study. APT MRI was conducted on a 3T clinical scanner and was obtained with 2 s saturation at 25 saturation frequency offsets ω =-6 to +6 ppm (step 0.5 ppm). δB0 maps were acquired separately for a point-by-point δB0 correction. APT signal intensity (SI) was defined as magnetization transfer asymmetry at 3.5 ppm: magnetization transfer ratio (MTR)asym = (S[-3.5 ppm]-S [+3.5 ppm])/S0. Regions of interest were carefully placed by 2 neuroradiologists in solid parts within brain tumors. The APT SI was compared with World Health Organization grade, Ki-67 labeling index (LI), and cell density.ResultsThe mean APT SI values were 2.1 ± 0.4% in grade II gliomas (n = 8), 3.2 ± 0.9% in grade III gliomas (n = 10), and 4.1 ± 1.0% in grade IV gliomas (n = 18). Significant differences in APT intensity were observed between grades II and III (P <. 05) and grades III and IV (P <. 05), as well as between grades II and IV (P <. 001). There were positive correlations between APT SI and Ki-67 LI (P =. 01, R = 0.43) and between APT SI and cell density (P <. 05, R = 0.38). The gliomas with microscopic necrosis showed higher APT SI than those without necrosis (P <. 001).ConclusionsAPT imaging can predict the histopathological grades of adult diffuse gliomas.

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