Ammosamides A and B target myosin

Chambers C. Hughes, John B. MacMillan, Susana P. Gaudêncio, William Fenical, James J. La Clair

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

The protein target of the ammosamides, cytotoxic natural products from a marine-derived actinomycete, has been elucidated. An immunoaffinity fluorescent tag was used to construct a fluorescent molecular probe (see structure). First, uptake and localization of the probe in cells was visualized with fluorescence microscopy. The probe was then used to co-immunoprecipitate proteins that bound to the ammosamide core. Myosin was thus identified as a target. (Figure Presented).

Original languageEnglish (US)
Pages (from-to)728-732
Number of pages5
JournalAngewandte Chemie - International Edition
Volume48
Issue number4
DOIs
StatePublished - Jan 12 2009

Keywords

  • Actinomycetes
  • Cell cycle inhibitors
  • Mitosis
  • Myosin
  • Natural products

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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