AMP-activated protein kinase is required for induction of apoptosis and epithelial-to-mesenchymal transition

Xunde Wang, Xinchao Pan, Jianguo Song

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

AMP-activated protein kinase (AMPK) is a serine/threonine protein kinase which has been implicated in the regulation of cellular energy homeostasis. Relatively very little is known about its role in other cellular processes. We observed that AMPK-α can be activated by transforming growth factor-β1 (TGF-β1) in mouse hepatocytes. Inhibition of AMPK by Compound C, a selective AMPK-α inhibitor, inhibited TGF-β1-induced apoptosis and EMT in hepatocytes. In addition, overexpression of a dominant-negative form of AMPK-α subunit also suppressed TGF-β1-induced EMT and apoptosis in AML12 cells. Furthermore, inhibition of AMPK suppressed TGF-β1-induced Smad3 transcriptional activity. This study indicates that AMPK is able to modulate Smad3 transcriptional activity, which plays an important role in TGF-β1-induced apoptosis and EMT.

Original languageEnglish (US)
Pages (from-to)1790-1797
Number of pages8
JournalCellular Signalling
Volume22
Issue number11
DOIs
StatePublished - Nov 1 2010

Keywords

  • AMP-activated protein kinase
  • Apoptosis
  • Epithelial-to-mesenchymal transition
  • TGF-beta

ASJC Scopus subject areas

  • Cell Biology

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