Amplification of the effects of magnetization exchange by 31P band inversion for measuring adenosine triphosphate synthesis rates in human skeletal muscle

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10 Citations (Scopus)

Abstract

Purpose The goal of this study was to amplify the effects of magnetization exchange between γ-adenosine triphosphate (ATP) and inorganic phosphate (Pi) for evaluation of ATP synthesis rates in human skeletal muscle. Methods The strategy works by simultaneously inverting the 31P resonances of phosphocreatine (PCR) and ATP using a wide bandwidth, adiabatic inversion radiofrequency pulse followed by observing dynamic changes in intensity of the noninverted Pi signal versus the delay time between the inversion and observation pulses. This band inversion technique significantly delays recovery of γ-ATP magnetization; consequently, the exchange reaction, Pi 虠 γ-ATP, is readily detected and easily analyzed. Results The ATP synthesis rate measured from high-quality spectral data using this method was 0.073 ± 0.011 s-1 in resting human skeletal muscle (N = 10). The T1 of Pi was 6.93 ± 1.90 s, consistent with the intrinsic T1 of Pi at this field. The apparent T1 of γ-ATP was 4.07 ± 0.32 s, about two-fold longer than its intrinsic T1 due to storage of magnetization in PCR. Conclusion Band inversion provides an effective method to amplify the effects of magnetization transfer between γ-ATP and Pi. The resulting data can be easily analyzed to obtain the ATP synthesis rate using a two-site exchange model. Magn Reson Med 74:1505-1514, 2015.

Original languageEnglish (US)
Pages (from-to)1505-1514
Number of pages10
JournalMagnetic Resonance in Medicine
Volume74
Issue number6
DOIs
StatePublished - Dec 1 2015

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Skeletal Muscle
Adenosine Triphosphate
Phosphocreatine
Adenine Nucleotides
Phosphates
Observation

Keywords

  • ATP
  • chemical exchange
  • magnetization transfer
  • skeletal muscle
  • T1 relaxation time

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

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title = "Amplification of the effects of magnetization exchange by 31P band inversion for measuring adenosine triphosphate synthesis rates in human skeletal muscle",
abstract = "Purpose The goal of this study was to amplify the effects of magnetization exchange between γ-adenosine triphosphate (ATP) and inorganic phosphate (Pi) for evaluation of ATP synthesis rates in human skeletal muscle. Methods The strategy works by simultaneously inverting the 31P resonances of phosphocreatine (PCR) and ATP using a wide bandwidth, adiabatic inversion radiofrequency pulse followed by observing dynamic changes in intensity of the noninverted Pi signal versus the delay time between the inversion and observation pulses. This band inversion technique significantly delays recovery of γ-ATP magnetization; consequently, the exchange reaction, Pi 虠 γ-ATP, is readily detected and easily analyzed. Results The ATP synthesis rate measured from high-quality spectral data using this method was 0.073 ± 0.011 s-1 in resting human skeletal muscle (N = 10). The T1 of Pi was 6.93 ± 1.90 s, consistent with the intrinsic T1 of Pi at this field. The apparent T1 of γ-ATP was 4.07 ± 0.32 s, about two-fold longer than its intrinsic T1 due to storage of magnetization in PCR. Conclusion Band inversion provides an effective method to amplify the effects of magnetization transfer between γ-ATP and Pi. The resulting data can be easily analyzed to obtain the ATP synthesis rate using a two-site exchange model. Magn Reson Med 74:1505-1514, 2015.",
keywords = "ATP, chemical exchange, magnetization transfer, skeletal muscle, T1 relaxation time",
author = "Jimin Ren and Sherry, {A. Dean} and Malloy, {Craig R.}",
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T1 - Amplification of the effects of magnetization exchange by 31P band inversion for measuring adenosine triphosphate synthesis rates in human skeletal muscle

AU - Ren, Jimin

AU - Sherry, A. Dean

AU - Malloy, Craig R.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Purpose The goal of this study was to amplify the effects of magnetization exchange between γ-adenosine triphosphate (ATP) and inorganic phosphate (Pi) for evaluation of ATP synthesis rates in human skeletal muscle. Methods The strategy works by simultaneously inverting the 31P resonances of phosphocreatine (PCR) and ATP using a wide bandwidth, adiabatic inversion radiofrequency pulse followed by observing dynamic changes in intensity of the noninverted Pi signal versus the delay time between the inversion and observation pulses. This band inversion technique significantly delays recovery of γ-ATP magnetization; consequently, the exchange reaction, Pi 虠 γ-ATP, is readily detected and easily analyzed. Results The ATP synthesis rate measured from high-quality spectral data using this method was 0.073 ± 0.011 s-1 in resting human skeletal muscle (N = 10). The T1 of Pi was 6.93 ± 1.90 s, consistent with the intrinsic T1 of Pi at this field. The apparent T1 of γ-ATP was 4.07 ± 0.32 s, about two-fold longer than its intrinsic T1 due to storage of magnetization in PCR. Conclusion Band inversion provides an effective method to amplify the effects of magnetization transfer between γ-ATP and Pi. The resulting data can be easily analyzed to obtain the ATP synthesis rate using a two-site exchange model. Magn Reson Med 74:1505-1514, 2015.

AB - Purpose The goal of this study was to amplify the effects of magnetization exchange between γ-adenosine triphosphate (ATP) and inorganic phosphate (Pi) for evaluation of ATP synthesis rates in human skeletal muscle. Methods The strategy works by simultaneously inverting the 31P resonances of phosphocreatine (PCR) and ATP using a wide bandwidth, adiabatic inversion radiofrequency pulse followed by observing dynamic changes in intensity of the noninverted Pi signal versus the delay time between the inversion and observation pulses. This band inversion technique significantly delays recovery of γ-ATP magnetization; consequently, the exchange reaction, Pi 虠 γ-ATP, is readily detected and easily analyzed. Results The ATP synthesis rate measured from high-quality spectral data using this method was 0.073 ± 0.011 s-1 in resting human skeletal muscle (N = 10). The T1 of Pi was 6.93 ± 1.90 s, consistent with the intrinsic T1 of Pi at this field. The apparent T1 of γ-ATP was 4.07 ± 0.32 s, about two-fold longer than its intrinsic T1 due to storage of magnetization in PCR. Conclusion Band inversion provides an effective method to amplify the effects of magnetization transfer between γ-ATP and Pi. The resulting data can be easily analyzed to obtain the ATP synthesis rate using a two-site exchange model. Magn Reson Med 74:1505-1514, 2015.

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