Amylin secretion from the rat pancreas and its selective loss after streptozotocin treatment

Atsushi Ogawa, Virginia Harris, Sara K. McCorkle, Roger H Unger, Kenneth L. Luskey

Research output: Contribution to journalArticlepeer-review

167 Scopus citations

Abstract

Amylin, a peptide copackaged with insulin in β-cell granules, was measured in the effluent of the perfused rat pancreas by means of a newly developed specific radioimmunoassay. Its secretion parallels that of insulin in response to 20 mM glucose, 10 mM arginine, or the combination thereof. The relative molar amount of secreted amylin was estimated to be 25-37% that of insulin. Treatment with a borderline diabetogenic dose of streptozotocin reduced amylin response without significantly changing the insulin response. A severely diabetogenic dose of streptozotocin totally abolished amylin release and markedly reduced insulin release. The selective impairment of amylin secretion in streptozotocin-treated rats could represent an early manifestation of β-cell depletion or injury.

Original languageEnglish (US)
Pages (from-to)973-976
Number of pages4
JournalJournal of Clinical Investigation
Volume85
Issue number3
DOIs
StatePublished - 1990

Keywords

  • Amyloid
  • Diabetes
  • Insulin
  • Islets
  • Radioimmunoassay

ASJC Scopus subject areas

  • Medicine(all)

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