Amylin secretion from the rat pancreas and its selective loss after streptozotocin treatment

Atsushi Ogawa, Virginia Harris, Sara K. McCorkle, Roger H Unger, Kenneth L. Luskey

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Amylin, a peptide copackaged with insulin in β-cell granules, was measured in the effluent of the perfused rat pancreas by means of a newly developed specific radioimmunoassay. Its secretion parallels that of insulin in response to 20 mM glucose, 10 mM arginine, or the combination thereof. The relative molar amount of secreted amylin was estimated to be 25-37% that of insulin. Treatment with a borderline diabetogenic dose of streptozotocin reduced amylin response without significantly changing the insulin response. A severely diabetogenic dose of streptozotocin totally abolished amylin release and markedly reduced insulin release. The selective impairment of amylin secretion in streptozotocin-treated rats could represent an early manifestation of β-cell depletion or injury.

Original languageEnglish (US)
Pages (from-to)973-976
Number of pages4
JournalJournal of Clinical Investigation
Volume85
Issue number3
StatePublished - Mar 1990

Fingerprint

Islet Amyloid Polypeptide
Streptozocin
Pancreas
Insulin
Radioimmunoassay
Arginine
Glucose
Peptides
Wounds and Injuries

Keywords

  • Amyloid
  • Diabetes
  • Insulin
  • Islets
  • Radioimmunoassay

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Amylin secretion from the rat pancreas and its selective loss after streptozotocin treatment. / Ogawa, Atsushi; Harris, Virginia; McCorkle, Sara K.; Unger, Roger H; Luskey, Kenneth L.

In: Journal of Clinical Investigation, Vol. 85, No. 3, 03.1990, p. 973-976.

Research output: Contribution to journalArticle

Ogawa, Atsushi ; Harris, Virginia ; McCorkle, Sara K. ; Unger, Roger H ; Luskey, Kenneth L. / Amylin secretion from the rat pancreas and its selective loss after streptozotocin treatment. In: Journal of Clinical Investigation. 1990 ; Vol. 85, No. 3. pp. 973-976.
@article{fa6b495abf3740818fc1a49f2163a09d,
title = "Amylin secretion from the rat pancreas and its selective loss after streptozotocin treatment",
abstract = "Amylin, a peptide copackaged with insulin in β-cell granules, was measured in the effluent of the perfused rat pancreas by means of a newly developed specific radioimmunoassay. Its secretion parallels that of insulin in response to 20 mM glucose, 10 mM arginine, or the combination thereof. The relative molar amount of secreted amylin was estimated to be 25-37{\%} that of insulin. Treatment with a borderline diabetogenic dose of streptozotocin reduced amylin response without significantly changing the insulin response. A severely diabetogenic dose of streptozotocin totally abolished amylin release and markedly reduced insulin release. The selective impairment of amylin secretion in streptozotocin-treated rats could represent an early manifestation of β-cell depletion or injury.",
keywords = "Amyloid, Diabetes, Insulin, Islets, Radioimmunoassay",
author = "Atsushi Ogawa and Virginia Harris and McCorkle, {Sara K.} and Unger, {Roger H} and Luskey, {Kenneth L.}",
year = "1990",
month = "3",
language = "English (US)",
volume = "85",
pages = "973--976",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "3",

}

TY - JOUR

T1 - Amylin secretion from the rat pancreas and its selective loss after streptozotocin treatment

AU - Ogawa, Atsushi

AU - Harris, Virginia

AU - McCorkle, Sara K.

AU - Unger, Roger H

AU - Luskey, Kenneth L.

PY - 1990/3

Y1 - 1990/3

N2 - Amylin, a peptide copackaged with insulin in β-cell granules, was measured in the effluent of the perfused rat pancreas by means of a newly developed specific radioimmunoassay. Its secretion parallels that of insulin in response to 20 mM glucose, 10 mM arginine, or the combination thereof. The relative molar amount of secreted amylin was estimated to be 25-37% that of insulin. Treatment with a borderline diabetogenic dose of streptozotocin reduced amylin response without significantly changing the insulin response. A severely diabetogenic dose of streptozotocin totally abolished amylin release and markedly reduced insulin release. The selective impairment of amylin secretion in streptozotocin-treated rats could represent an early manifestation of β-cell depletion or injury.

AB - Amylin, a peptide copackaged with insulin in β-cell granules, was measured in the effluent of the perfused rat pancreas by means of a newly developed specific radioimmunoassay. Its secretion parallels that of insulin in response to 20 mM glucose, 10 mM arginine, or the combination thereof. The relative molar amount of secreted amylin was estimated to be 25-37% that of insulin. Treatment with a borderline diabetogenic dose of streptozotocin reduced amylin response without significantly changing the insulin response. A severely diabetogenic dose of streptozotocin totally abolished amylin release and markedly reduced insulin release. The selective impairment of amylin secretion in streptozotocin-treated rats could represent an early manifestation of β-cell depletion or injury.

KW - Amyloid

KW - Diabetes

KW - Insulin

KW - Islets

KW - Radioimmunoassay

UR - http://www.scopus.com/inward/record.url?scp=0025262297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025262297&partnerID=8YFLogxK

M3 - Article

VL - 85

SP - 973

EP - 976

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 3

ER -