An actively retrotransposing, novel subfamily of mouse L1 elements

Thierry P. Naas, Ralph J. Deberardinis, John V. Moran, Eric M. Ostertag, Stephen F. Kingsmore, Michael F. Seldin, Yoshihide Hayashizaki, Sandra L. Martin, Haig H. Kazazian

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Retrotransposition of LINEs and other retroelements increases repetition in mammalian genomes and can cause deleterious mutations. Recent insertions of two full-length L1s, L1(spa) and L1(Orl), caused the disease phenotypes of the spastic and Orleans reeler mice respectively. Here we show that these two recently retrotransposed L1s are nearly identical in sequence, have two open reading frames and belong to a novel subfamily related to the ancient F subfamily. We have named this new subfamily T(F) (for transposable) and show that many full-length members of this family are present in the mouse genome. The T(F) 5' untranslated region has promoter activity, and T(F)-type RNA is abundant in cytoplasmic ribonucleoprotein particles, which are likely intermediates in retrotransposition. Both L1(spa) and L1(Orl) have reverse transcriptase activity in a yeast-based assay and retrotranspose at high frequency in cultured cells. Together, our data indicate that the T(F) subfamily of L1s contains a major class of mobile elements that is expanding in the mouse genome.

Original languageEnglish (US)
Pages (from-to)590-597
Number of pages8
JournalEMBO Journal
Volume17
Issue number2
DOIs
StatePublished - Jan 15 1998

Fingerprint

Long Interspersed Nucleotide Elements
Genes
Genome
Neurologic Mutant Mice
Retroelements
Ribonucleoproteins
Muscle Spasticity
5' Untranslated Regions
RNA-Directed DNA Polymerase
Yeast
Open Reading Frames
Cultured Cells
Assays
Yeasts
Cells
RNA
Phenotype
Mutation

Keywords

  • L1
  • LINEs
  • Pseudogene
  • Retrotransposition
  • Reverse transcriptase

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Naas, T. P., Deberardinis, R. J., Moran, J. V., Ostertag, E. M., Kingsmore, S. F., Seldin, M. F., ... Kazazian, H. H. (1998). An actively retrotransposing, novel subfamily of mouse L1 elements. EMBO Journal, 17(2), 590-597. https://doi.org/10.1093/emboj/17.2.590

An actively retrotransposing, novel subfamily of mouse L1 elements. / Naas, Thierry P.; Deberardinis, Ralph J.; Moran, John V.; Ostertag, Eric M.; Kingsmore, Stephen F.; Seldin, Michael F.; Hayashizaki, Yoshihide; Martin, Sandra L.; Kazazian, Haig H.

In: EMBO Journal, Vol. 17, No. 2, 15.01.1998, p. 590-597.

Research output: Contribution to journalArticle

Naas, TP, Deberardinis, RJ, Moran, JV, Ostertag, EM, Kingsmore, SF, Seldin, MF, Hayashizaki, Y, Martin, SL & Kazazian, HH 1998, 'An actively retrotransposing, novel subfamily of mouse L1 elements', EMBO Journal, vol. 17, no. 2, pp. 590-597. https://doi.org/10.1093/emboj/17.2.590
Naas TP, Deberardinis RJ, Moran JV, Ostertag EM, Kingsmore SF, Seldin MF et al. An actively retrotransposing, novel subfamily of mouse L1 elements. EMBO Journal. 1998 Jan 15;17(2):590-597. https://doi.org/10.1093/emboj/17.2.590
Naas, Thierry P. ; Deberardinis, Ralph J. ; Moran, John V. ; Ostertag, Eric M. ; Kingsmore, Stephen F. ; Seldin, Michael F. ; Hayashizaki, Yoshihide ; Martin, Sandra L. ; Kazazian, Haig H. / An actively retrotransposing, novel subfamily of mouse L1 elements. In: EMBO Journal. 1998 ; Vol. 17, No. 2. pp. 590-597.
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