An adenosine-mediated glial-neuronal circuit for homeostatic sleep

Theresa Bjorness, Nicholas Dale, Gabriel Mettlach, Alex Sonneborn, Bogachan Sahin, Allen A. Fienberg, Masashi Yanagisawa, James A Bibb, Robert Greene

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Sleep homeostasis reflects a centrally mediated drive for sleep, which increases during waking and resolves during subsequent sleep. Here we demonstrate that mice deficient for glial adenosine kinase (AdK), the primary metabolizing enzyme for adenosine (Ado), exhibit enhanced expression of this homeostatic drive by three independent measures: (1) increased rebound of slow-wave activity; (2) increased consolidation of slow-wave sleep; and (3) increased time constant of slow-wave activity decay during an average slow-wave sleep episode, proposed and validated here as a new index for homeostatic sleep drive. Conversely, mice deficient for the neuronal adenosine A1 receptor exhibit significantly decreased sleep drive as judged by these same indices. Neuronal knock-out of AdK did not influence homeostatic sleep need. Together, these findings implicate a glial-neuronal circuit mediated by intercellular Ado, controlling expression of homeostatic sleep drive. Because AdK is tightly regulated by glial metabolic state, our findings suggest a functional link between cellular metabolism and sleep homeostasis.

Original languageEnglish (US)
Pages (from-to)3709-3721
Number of pages13
JournalJournal of Neuroscience
Volume36
Issue number13
DOIs
StatePublished - Mar 30 2016

Fingerprint

Neuroglia
Adenosine
Sleep
Adenosine Kinase
Homeostasis
Adenosine A1 Receptors
Drive
Enzymes

Keywords

  • Adenosine
  • Adenosine kinase
  • Delta power
  • Glia
  • Sleep
  • SWS

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

An adenosine-mediated glial-neuronal circuit for homeostatic sleep. / Bjorness, Theresa; Dale, Nicholas; Mettlach, Gabriel; Sonneborn, Alex; Sahin, Bogachan; Fienberg, Allen A.; Yanagisawa, Masashi; Bibb, James A; Greene, Robert.

In: Journal of Neuroscience, Vol. 36, No. 13, 30.03.2016, p. 3709-3721.

Research output: Contribution to journalArticle

Bjorness T, Dale N, Mettlach G, Sonneborn A, Sahin B, Fienberg AA et al. An adenosine-mediated glial-neuronal circuit for homeostatic sleep. Journal of Neuroscience. 2016 Mar 30;36(13):3709-3721. https://doi.org/10.1523/JNEUROSCI.3906-15.2016
Bjorness, Theresa ; Dale, Nicholas ; Mettlach, Gabriel ; Sonneborn, Alex ; Sahin, Bogachan ; Fienberg, Allen A. ; Yanagisawa, Masashi ; Bibb, James A ; Greene, Robert. / An adenosine-mediated glial-neuronal circuit for homeostatic sleep. In: Journal of Neuroscience. 2016 ; Vol. 36, No. 13. pp. 3709-3721.
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