An agonist specific effect of guanine nucleotides on binding to the beta adrenergic receptor

M. E. Maguire, P. M. Van Arsdale, A. G. Gilman

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

The authors have previously characterized the binding of [125I] iodohydroxybenzylpindolol to beta adrenergic receptor sites in two clonal lines of cultured cells. They now report that whereas guanine nucleotides have no effect on the binding of this iodinated antagonist, GTP, GDP, and guanylyl imidodiphosphate all impair the ability of beta adrenergic agonists to inhibit the binding of [125I]iodohydroxybenzylpindolol. The nucleotides have no effect on the ability of other beta adrenergic antagonists to inhibit binding. Thus, with this method, beta adrenergic agonists and antagonists can be distinguished by means of binding alone; no assessment of 'effect' need be made.

Original languageEnglish (US)
Pages (from-to)335-339
Number of pages5
JournalMolecular Pharmacology
Volume12
Issue number2
StatePublished - 1976

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Adrenergic beta-Agonists
Guanine Nucleotides
Receptors, Adrenergic, beta
Guanylyl Imidodiphosphate
Adrenergic beta-Antagonists
Adrenergic Antagonists
Needs Assessment
Guanosine Triphosphate
Cultured Cells
Nucleotides
iodohydroxybenzylpindolol

ASJC Scopus subject areas

  • Pharmacology

Cite this

Maguire, M. E., Van Arsdale, P. M., & Gilman, A. G. (1976). An agonist specific effect of guanine nucleotides on binding to the beta adrenergic receptor. Molecular Pharmacology, 12(2), 335-339.

An agonist specific effect of guanine nucleotides on binding to the beta adrenergic receptor. / Maguire, M. E.; Van Arsdale, P. M.; Gilman, A. G.

In: Molecular Pharmacology, Vol. 12, No. 2, 1976, p. 335-339.

Research output: Contribution to journalArticle

Maguire, ME, Van Arsdale, PM & Gilman, AG 1976, 'An agonist specific effect of guanine nucleotides on binding to the beta adrenergic receptor', Molecular Pharmacology, vol. 12, no. 2, pp. 335-339.
Maguire, M. E. ; Van Arsdale, P. M. ; Gilman, A. G. / An agonist specific effect of guanine nucleotides on binding to the beta adrenergic receptor. In: Molecular Pharmacology. 1976 ; Vol. 12, No. 2. pp. 335-339.
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