An algal nucleus-encoded subunit of mitochondrial ATP synthase rescues a defect in the analogous human mitochondrial-encoded subunit

Joseline Ojaimi, Junmin Pan, Sumana Santra, William J. Snell, Eric A. Schon

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Unlike most organisms, the mitochondrial DNA (mtDNA) of Chlamydomonas reinhardtii, a green alga, does not encode subunit 6 of F0F1-ATP synthase. We hypothesized that C. reinhardtii ATPase 6 is nucleus encoded and identified cDNAs and a single-copy nuclear gene specifying this subunit (CrATP6, with eight exons, four of which encode a mitochondrial targeting signal). Although the algal and human ATP6 genes are in different subcellular compartments and the encoded polypeptides are highly diverged, their secondary structures are remarkably similar. When CrATP6 was expressed in human cells, a significant amount of the precursor polypeptide was targeted to mitochondria, the mitochondrial targeting signal was cleaved within the organelle, and the mature polypeptide was assembled into human ATP synthase. In spite of the evolutionary distance between algae and mammals, C. reinhardtii ATPase 6 functioned in human cells, because deficiencies in both cell viability and ATP synthesis in transmitochondrial cell lines harboring a pathogenic mutation in the human mtDNA-encoded ATP6 gene were overcome by expression of CrATP6. The ability to express a nucleus-encoded version of a mammalian mtDNA-encoded protein may provide a way to import other highly hydrophobic proteins into mitochondria and could serve as the basis for a gene therapy approach to treat human mitochondrial diseases.

Original languageEnglish (US)
Pages (from-to)3836-3844
Number of pages9
JournalMolecular Biology of the Cell
Volume13
Issue number11
DOIs
StatePublished - Nov 1 2002

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Mitochondrial Proton-Translocating ATPases
Chlamydomonas reinhardtii
Mitochondrial DNA
Peptides
Adenosine Triphosphatases
Mitochondria
Adenosine Triphosphate
Genes
Mitochondrial Diseases
Chlorophyta
Genetic Therapy
Organelles
Mammals
Exons
Cell Survival
Proteins
Complementary DNA
Cell Line
Mutation

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

An algal nucleus-encoded subunit of mitochondrial ATP synthase rescues a defect in the analogous human mitochondrial-encoded subunit. / Ojaimi, Joseline; Pan, Junmin; Santra, Sumana; Snell, William J.; Schon, Eric A.

In: Molecular Biology of the Cell, Vol. 13, No. 11, 01.11.2002, p. 3836-3844.

Research output: Contribution to journalArticle

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