An alternative pluripotent state confers interspecies chimaeric competency

Jun Wu, Daiji Okamura, Mo Li, Keiichiro Suzuki, Chongyuan Luo, Li Ma, Yupeng He, Zhongwei Li, Chris Benner, Isao Tamura, Marie N. Krause, Joseph R. Nery, Tingting Du, Zhuzhu Zhang, Tomoaki Hishida, Yuta Takahashi, Emi Aizawa, Na Young Kim, Jeronimo Lajara, Pedro GuillenJosep M. Campistol, Concepcion Rodriguez Esteban, Pablo J. Ross, Alan Saghatelian, Bing Ren, Joseph R. Ecker, Juan Carlos Izpisua Belmonte

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Pluripotency, the ability to generate any cell type of the body, is an evanescent attribute of embryonic cells. Transitory pluripotent cells can be captured at different time points during embryogenesis and maintained as embryonic stem cells or epiblast stem cells in culture. Since ontogenesis is a dynamic process in both space and time, it seems counterintuitive that these two temporal states represent the full spectrum of organismal pluripotency. Here we show that by modulating culture parameters, a stem-cell type with unique spatial characteristics and distinct molecular and functional features, designated as region-selective pluripotent stem cells (rsPSCs), can be efficiently obtained from mouse embryos and primate pluripotent stem cells, including humans. The ease of culturing and editing the genome of human rsPSCs offers advantages for regenerative medicine applications. The unique ability of human rsPSCs to generate post-implantation interspecies chimaeric embryos may facilitate our understanding of early human development and evolution.

Original languageEnglish (US)
Pages (from-to)316-321
Number of pages6
JournalNature
Volume521
Issue number7552
DOIs
StatePublished - May 21 2015
Externally publishedYes

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Pluripotent Stem Cells
Aptitude
Stem Cells
Embryonic Structures
Germ Layers
Regenerative Medicine
Human Development
Human Genome
Embryonic Stem Cells
Primates
Embryonic Development
Cell Culture Techniques

ASJC Scopus subject areas

  • General

Cite this

Wu, J., Okamura, D., Li, M., Suzuki, K., Luo, C., Ma, L., ... Belmonte, J. C. I. (2015). An alternative pluripotent state confers interspecies chimaeric competency. Nature, 521(7552), 316-321. https://doi.org/10.1038/nature14413

An alternative pluripotent state confers interspecies chimaeric competency. / Wu, Jun; Okamura, Daiji; Li, Mo; Suzuki, Keiichiro; Luo, Chongyuan; Ma, Li; He, Yupeng; Li, Zhongwei; Benner, Chris; Tamura, Isao; Krause, Marie N.; Nery, Joseph R.; Du, Tingting; Zhang, Zhuzhu; Hishida, Tomoaki; Takahashi, Yuta; Aizawa, Emi; Kim, Na Young; Lajara, Jeronimo; Guillen, Pedro; Campistol, Josep M.; Esteban, Concepcion Rodriguez; Ross, Pablo J.; Saghatelian, Alan; Ren, Bing; Ecker, Joseph R.; Belmonte, Juan Carlos Izpisua.

In: Nature, Vol. 521, No. 7552, 21.05.2015, p. 316-321.

Research output: Contribution to journalArticle

Wu, J, Okamura, D, Li, M, Suzuki, K, Luo, C, Ma, L, He, Y, Li, Z, Benner, C, Tamura, I, Krause, MN, Nery, JR, Du, T, Zhang, Z, Hishida, T, Takahashi, Y, Aizawa, E, Kim, NY, Lajara, J, Guillen, P, Campistol, JM, Esteban, CR, Ross, PJ, Saghatelian, A, Ren, B, Ecker, JR & Belmonte, JCI 2015, 'An alternative pluripotent state confers interspecies chimaeric competency', Nature, vol. 521, no. 7552, pp. 316-321. https://doi.org/10.1038/nature14413
Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L et al. An alternative pluripotent state confers interspecies chimaeric competency. Nature. 2015 May 21;521(7552):316-321. https://doi.org/10.1038/nature14413
Wu, Jun ; Okamura, Daiji ; Li, Mo ; Suzuki, Keiichiro ; Luo, Chongyuan ; Ma, Li ; He, Yupeng ; Li, Zhongwei ; Benner, Chris ; Tamura, Isao ; Krause, Marie N. ; Nery, Joseph R. ; Du, Tingting ; Zhang, Zhuzhu ; Hishida, Tomoaki ; Takahashi, Yuta ; Aizawa, Emi ; Kim, Na Young ; Lajara, Jeronimo ; Guillen, Pedro ; Campistol, Josep M. ; Esteban, Concepcion Rodriguez ; Ross, Pablo J. ; Saghatelian, Alan ; Ren, Bing ; Ecker, Joseph R. ; Belmonte, Juan Carlos Izpisua. / An alternative pluripotent state confers interspecies chimaeric competency. In: Nature. 2015 ; Vol. 521, No. 7552. pp. 316-321.
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