An apical membrane Na+/H+ exchanger isoform, NHE-3, is present in the rat epididymal epithelium

Kari Kaunisto, Orson W. Moe, Markku Pelto-Huikko, Martin Traebert, Hannu Rajaniemi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

An acidic milieu is required for sperm maturation and for keeping sperm quiescent during storage in the cauda epididymidis. Previous studies have implicated a Na+/H+ exchanger (NHE) in epididymal acidification together with carbonic anhydrase (CA) and vacuolar proton adenosine triphosphatase (H+-ATPase). The present studies were undertaken to discover whether the NHE isoform involved is NHE-3, which is known to mediate Na+ and HCO3 - absorption in renal tubules. Using the reverse transcription polymerase chain reaction technique (RT-PCR), Northern blot analysis and in situ hybridization, NHE-3 mRNA was detected mainly in the cauda epididymis and to a lesser extent in other regions of the epididymis. Immunohistochemical studies showed that NHE-3 was present in the apical membranes of the epithelial principal cells and confirmed that its expression is strongest in the cauda region, decreasing towards the more proximal regions. Immunoblotting showed a similar expression pattern. These results demonstrate that NHE-3 is expressed in the rat epididymal duct with strongest expression in its cauda region. These findings are thus consistent with the possibility that NHE-3 in the epididymal duct is involved in luminal Na+ and/or HCO3 - absorption, as in the renal proximal tubule, and thereby in the regulation of sperm motility and maturation.

Original languageEnglish (US)
Pages (from-to)230-236
Number of pages7
JournalPflugers Archiv European Journal of Physiology
Volume442
Issue number2
DOIs
StatePublished - 2001

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Sodium-Hydrogen Antiporter
Rats
Protein Isoforms
Epithelium
Membranes
Sperm Maturation
Epididymis
Ducts
Proximal Kidney Tubule
Proton-Translocating ATPases
Carbonic Anhydrases
Acidification
Sperm Motility
Polymerase chain reaction
Transcription
Immunoblotting
Northern Blotting
Reverse Transcription
In Situ Hybridization
Adenosine Triphosphatases

Keywords

  • Bicarbonate
  • Immunoblotting
  • Immunohistochemistry
  • Northern blotting
  • Sodium-hydrogen antiporter
  • Sperm motility

ASJC Scopus subject areas

  • Physiology

Cite this

An apical membrane Na+/H+ exchanger isoform, NHE-3, is present in the rat epididymal epithelium. / Kaunisto, Kari; Moe, Orson W.; Pelto-Huikko, Markku; Traebert, Martin; Rajaniemi, Hannu.

In: Pflugers Archiv European Journal of Physiology, Vol. 442, No. 2, 2001, p. 230-236.

Research output: Contribution to journalArticle

Kaunisto, Kari ; Moe, Orson W. ; Pelto-Huikko, Markku ; Traebert, Martin ; Rajaniemi, Hannu. / An apical membrane Na+/H+ exchanger isoform, NHE-3, is present in the rat epididymal epithelium. In: Pflugers Archiv European Journal of Physiology. 2001 ; Vol. 442, No. 2. pp. 230-236.
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