Abstract
In this report, we describe an assay for screening monoclonal antibodies for their cytotoxic potential as ricin A chain-containing immunotoxins. The assay involves treating cells with dilutions of the test antibody followed by a Fab fragment of a secondary antibody coupled to ricin A chain (“indirect assay”). The cytotoxicity of the indirect assay is compared to that of the direct assay where the monoclonal antibody is coupled to ricin A chain. Indirect and direct assays were carried out using 14 antibodies and a panel of 8 human and mouse cell types. The two assays showed virtually 100% correlation. The indirect assay, therefore, predicts the potency of a given monoclonal antibody to make an effective immunotoxin and should be useful in screening monoclonal antibodies for use as immunotoxins.
Original language | English (US) |
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Pages (from-to) | 1119-1123 |
Number of pages | 5 |
Journal | Cancer research |
Volume | 48 |
Issue number | 5 |
State | Published - 1988 |
ASJC Scopus subject areas
- Oncology
- Cancer Research