An embryonic culture system for the investigation of striatal medium spiny neuron dendritic spine development and plasticity

Rachel D. Penrod, Saïd Kourrich, Esther Kearney, Mark J. Thomas, Lorene M. Lanier

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Dendritic spines of striatal Medium Spiny Neurons (MSNs) receive converging dopaminergic and glutamatergic inputs. These spines undergo experience-dependent structural plasticity following repeated drug administration and during disease states like Huntington's and Parkinson's. Thus, understanding the molecular mechanisms leading to structural plasticity is an important step toward establishing a clear relationship between spine structure and function, and will ultimately contribute to understanding how changes in dendritic spine structure relate to behaviors or diseases. One major difficulty faced when studying MSN development is the lack of a detailed, standardized in vitro model system that produces MSNs with in vivo-like morphologies. For example, unlike cultured pyramidal neurons, MSNs grown in mono-cultures display stunted dendritic arborization and fail to develop a full cohort of mature dendritic spines. Here we report the generation of an embryonic mouse cortical-striatal co-culture that generates high cell yields from a single embryo. Unlike MSNs in striatal mono-culture, MSNs in co-culture develop in vivo-like morphologies and high densities of dendritic spines. Morphological identification of co-cultured MSNs expressing a soluble fluorescent protein can be confirmed by immunochemical detection of DARPP-32 (Dopamine and cyclic AMP regulated phospho. protein of 32. kDa). Additionally, co-cultured MSN spines contain PSD-95 puncta and are apposed to SV2 puncta, indicating the spines express synaptic machinery. Finally, whole-cell recordings of co-cultured MSNs exhibit higher mEPSC frequency compared to mono-cultured MSNs, suggesting that the spines are functionally mature. These studies establish that this co-culture system is suitable for studying the morphological and physiological development and function of MSN dendritic spines.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalJournal of Neuroscience Methods
Issue number1
StatePublished - Aug 30 2011


  • Cortex
  • Dendritic spines
  • Medium spiny neurons
  • Primary cell culture
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)


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