An endocrine-specific element is an integral component of an exocrine-specific pancreatic enhancer

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We have, analyzed the function of individual elements of the elastase I transcriptional enhancer in transgenic animals. This pancreas-specific enhancer comprises three functional elements, one of which (the B element) plays a dual role. Within the context of the enhancer, the B element contributes to appropriate acinar cell expression. However, when separated from the other enhancer components, the B element selectively directs transcription in islet cells of transgenic animals. This islet-specific activity is normally suppressed by an upstream represser domain. The B element binds a novel islet-specific factor, and similar B-like elements are present in other pancreatic genes, both exocrine and endocrine specific. We suggest that a principal role of this transcriptional element and its associated factors is to activate many pancreatic genes as part of the program of pancreatic determination prior to the divergence of the acinar and islet cell lineages.

Original languageEnglish (US)
Pages (from-to)774-786
Number of pages13
JournalGenes and Development
Volume7
Issue number5
StatePublished - 1993

Fingerprint

Genetically Modified Animals
Acinar Cells
Islets of Langerhans
Pancreatic Elastase
Cell Lineage
Genes
Pancreas
3'-(1-butylphosphoryl)adenosine

Keywords

  • Cell specificity
  • Elastase gene
  • Islet of Langerhans
  • Transcription element
  • Transcription factor
  • Transgenic mice

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

An endocrine-specific element is an integral component of an exocrine-specific pancreatic enhancer. / Kruse, Fred; Rose, Scott D.; Swift, Galvin H; Hammer, Robert E; MacDonald, Raymond J.

In: Genes and Development, Vol. 7, No. 5, 1993, p. 774-786.

Research output: Contribution to journalArticle

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