An Endothelial-to-Adipocyte Extracellular Vesicle Axis Governed by Metabolic State

Clair Crewe, Nolwenn Joffin, Joseph M. Rutkowski, Min Kim, Fang Zhang, Dwight A. Towler, Ruth Gordillo, Philipp E. Scherer

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

We have uncovered the existence of extracellular vesicle (EV)-mediated signaling between cell types within the adipose tissue (AT) proper. This phenomenon became evident in our attempts at generating an adipocyte-specific knockout of caveolin 1 (cav1) protein. Although we effectively ablated the CAV1 gene in adipocytes, cav1 protein remained abundant. With the use of newly generated mouse models, we show that neighboring endothelial cells (ECs) transfer cav1-containing EVs to adipocytes in vivo, which reciprocate by releasing EVs to ECs. AT-derived EVs contain proteins and lipids capable of modulating cellular signaling pathways. Furthermore, this mechanism facilitates transfer of plasma constituents from ECs to the adipocyte. The transfer event is physiologically regulated by fasting/refeeding and obesity, suggesting EVs participate in the tissue response to changes in the systemic nutrient state. This work offers new insights into the complex signaling mechanisms that exist among adipocytes, stromal vascular cells, and, potentially, distal organs. Extracellular vesicles exchange protein and lipid signals between endothelial cells and adipocytes conveying information about nutrient state changes from the blood in adipose tissues.

Original languageEnglish (US)
Pages (from-to)695-708.e13
JournalCell
Volume175
Issue number3
DOIs
StatePublished - Oct 18 2018

Keywords

  • adipose tissue
  • caveolin 1
  • endothelial cells
  • exosome
  • extracellular vesicle
  • fasting
  • glucagon
  • metabolism
  • obesity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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