TY - JOUR
T1 - An essential role for mef2c in the cortical response to loss of sleep in mice
AU - Bjorness, Theresa E.
AU - Kulkarni, Ashwinikumar
AU - Rybalchenko, Volodymyr
AU - Suzuki, Ayako
AU - Bridges, Catherine
AU - Harrington, Adam J.
AU - Cowan, Christopher W.
AU - Takahashi, Joseph S.
AU - Konopka, Genevieve
AU - Greene, Robert W.
N1 - Funding Information:
We wish to thank Mr. To Thai, Ms. Lilian Zhan and Dr. Busra Goksu for technical assistance and Dr. Stefano Berto for discussions about genomic analyses. Funding: This study was funded by NIH grant R01 MH080297 and WPI program for IIIS to R.W.G.; NIH grants RO1 MH102603, DC014702 and the James S. McDonnell Foundation 21st Century Science Initiative in Understanding Human Cognition ? Scholar Award (220020467) to G.K.; NIH grants R01 AG045795, R01 NS 106657 to J.S.T.; J.S.T is an Investigator in the Howard Hughes Medical Institute; NIH grant RO1 MH111464 to CWC and F30 HD098893 to CMB. The contents do not represent the views of the U.S. Department of Veterans Affairs or the United States Government.
Publisher Copyright:
© 2020, eLife Sciences Publications Ltd. All rights reserved.
PY - 2020/8
Y1 - 2020/8
N2 - Neuronal activity and gene expression in response to the loss of sleep can provide a window into the enigma of sleep function. Sleep loss is associated with brain differential gene expression, an increase in pyramidal cell mEPSC frequency and amplitude, and a characteristic rebound and resolution of slow wave sleep-slow wave activity (SWS-SWA). However, the molecular mechanism(s) mediating the sleep loss response are not well understood. We show that sleeploss regulates MEF2C phosphorylation, a key mechanism regulating MEF2C transcriptional activity, and that MEF2C function in postnatal excitatory forebrain neurons is required for the biological events in response to sleep loss in C57BL/6J mice. These include altered gene expression, the increase and recovery of synaptic strength, and the rebound and resolution of SWS-SWA, which implicate MEF2C as an essential regulator of sleep function. One Sentence Summary: MEF2C is critical to the response to sleep loss.
AB - Neuronal activity and gene expression in response to the loss of sleep can provide a window into the enigma of sleep function. Sleep loss is associated with brain differential gene expression, an increase in pyramidal cell mEPSC frequency and amplitude, and a characteristic rebound and resolution of slow wave sleep-slow wave activity (SWS-SWA). However, the molecular mechanism(s) mediating the sleep loss response are not well understood. We show that sleeploss regulates MEF2C phosphorylation, a key mechanism regulating MEF2C transcriptional activity, and that MEF2C function in postnatal excitatory forebrain neurons is required for the biological events in response to sleep loss in C57BL/6J mice. These include altered gene expression, the increase and recovery of synaptic strength, and the rebound and resolution of SWS-SWA, which implicate MEF2C as an essential regulator of sleep function. One Sentence Summary: MEF2C is critical to the response to sleep loss.
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U2 - 10.7554/ELIFE.58331
DO - 10.7554/ELIFE.58331
M3 - Article
C2 - 32851972
AN - SCOPUS:85090636712
VL - 9
SP - 1
EP - 46
JO - eLife
JF - eLife
SN - 2050-084X
ER -