An improved and simplified radioisotopic assay for the determination of free and esterified carnitine

J. D. McGarry, D. W. Foster

Research output: Contribution to journalArticle

486 Scopus citations

Abstract

The radioisotopic assay for carnitine first described by Cederblad and Lindstedt and modified by Bohmer et al has been improved and simplified. As a result, the assay yields a linear response over a wide range of carnitine concentrations without the need for excessive amounts of labeled acetyl CoA. In addition, it will measure very small quantities of carnitine even in the presence of excess acylcarnitine. The method allows rapid determination of free and esterified carnitine in small volumes of plasma (50 μl is sufficient) without the need for prior deproteinization of the samples.

Original languageEnglish (US)
Pages (from-to)277-281
Number of pages5
JournalJournal of lipid research
Volume17
Issue number3
StatePublished - Dec 1 1976

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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