An improved chemical ionization assay for mevalonic acid

Georg F. Hoffmann, Lawrence Sweetman

Research output: Contribution to journalArticlepeer-review

Abstract

The determination of mevalonic acid by gas chromatography/mass spectrometry (GC/MS) is complicated by poor extractability and multiple product formation. Reliable quantification down to physiological levels was achieved by isolating mevalonic acid by liquid partition chromatography and quantifying the underivatized lactone by means of ammonia chemical ionization, selected ion monitoring, capillary GC/MS. (2H3)mevalonic acid served as the internal standard. The method appears to be suitable for the study of mevalonic aciduria as well as the evaluation of different therapeutic regimens in patients with hypercholesterolaemia.

Original languageEnglish (US)
Pages (from-to)517-519
Number of pages3
JournalBiological Mass Spectrometry
Volume19
Issue number8
DOIs
StatePublished - Aug 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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