TY - JOUR
T1 - An in situ benzoylation-dispersive liquid-liquid microextraction method based on solidification of floating organic droplets for determination of biogenic amines by liquid chromatography-ultraviolet analysis
AU - Jia, Shaodong
AU - Ryu, Yeonsuk
AU - Kwon, Sung Won
AU - Lee, Jeongmi
N1 - Funding Information:
This work was supported by the SEOK CHUN Research Fund, Sungkyunkwan University, 2010 and a grant from the Basic Science Research Program (No. 2011-0024225 ) of the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (MEST), Republic of Korea .
PY - 2013/3/22
Y1 - 2013/3/22
N2 - A novel analytical method consisting of in situ derivatization combined with liquid phase microextraction followed by liquid chromatography-ultraviolet detection (LC-UV) was developed to determine the biogenic amines (BAs) of alcoholic beverages. Nine BAs (putrescine, cadaverine, 1,3-diaminopropane, tryptamine, phenylethylamine, spermidine, spermine, histamine, and tyramine) were derivatized in situ with benzoyl chloride, extracted by dispersive liquid-liquid microextraction based on solidification of floating organic droplets (DLLME-SFO), and then chromatographed by LC-UV. Factors influencing the derivatization and extraction efficiency were optimized, including the reaction buffer pH and concentration, amount of derivatization reagent, reaction time, types and volumes of extraction and dispersive solvents, and extraction time. Under the optimized conditions, the method was linear over 0.05-8.0μgmL-1 with an r2≥0.992 and exhibited intra- and inter-day precision less than 8.8% and 11.5%, respectively. The limit of detection ranged between 0.005 and 0.01μgmL-1. The developed method using a basic LC-UV system is sensitive, rapid, convenient, green, and cost-effective. Moreover, it is versatile and practical for the analysis of BAs, as demonstrated by the successful application in four different types of popular alcoholic beverages (white wine, red wine, rice wine, and beer).
AB - A novel analytical method consisting of in situ derivatization combined with liquid phase microextraction followed by liquid chromatography-ultraviolet detection (LC-UV) was developed to determine the biogenic amines (BAs) of alcoholic beverages. Nine BAs (putrescine, cadaverine, 1,3-diaminopropane, tryptamine, phenylethylamine, spermidine, spermine, histamine, and tyramine) were derivatized in situ with benzoyl chloride, extracted by dispersive liquid-liquid microextraction based on solidification of floating organic droplets (DLLME-SFO), and then chromatographed by LC-UV. Factors influencing the derivatization and extraction efficiency were optimized, including the reaction buffer pH and concentration, amount of derivatization reagent, reaction time, types and volumes of extraction and dispersive solvents, and extraction time. Under the optimized conditions, the method was linear over 0.05-8.0μgmL-1 with an r2≥0.992 and exhibited intra- and inter-day precision less than 8.8% and 11.5%, respectively. The limit of detection ranged between 0.005 and 0.01μgmL-1. The developed method using a basic LC-UV system is sensitive, rapid, convenient, green, and cost-effective. Moreover, it is versatile and practical for the analysis of BAs, as demonstrated by the successful application in four different types of popular alcoholic beverages (white wine, red wine, rice wine, and beer).
KW - Alcoholic beverage
KW - Benzoylation
KW - Biogenic amine
KW - Dispersive liquid-liquid microextraction
KW - In situ derivatization
KW - Solidification of floating organic droplets
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U2 - 10.1016/j.chroma.2013.01.041
DO - 10.1016/j.chroma.2013.01.041
M3 - Article
C2 - 23415468
AN - SCOPUS:84875367703
SN - 0021-9673
VL - 1282
SP - 1
EP - 10
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -