An integrated electrical enzyme-linked immunosorbent assay (E2LISA) for point-of-care diagnosis of tuberculosis using glycan biomarkers

Aniruddh Sarkar, Lenette L. Lu, Jongyoon Han, Galit Alter

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present an integrated electrical enzyme-linked immunosorbent assay chip for point-of-care (POC) diagnosis of tuberculosis (TB) using novel antigen-specific antibody glycosylation biomarkers. We developed assays for sensitive electrical detection of antigen-specific antibodies and their associated antibody glycans using enzymatic silver metallization on microelectrode arrays and integrated these with microfluidic serial dilution to enable measurement of binding curves from small sample volumes (~1uL). We used this chip to analyze patient sera and accurately distinguish latent TB infection (LTBI) and active TB (ATB) disease.

Original languageEnglish (US)
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages5-6
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - Jan 1 2020
Externally publishedYes
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • Electrical detection
  • ELISA
  • Point-of-care diagnostics
  • Tuberculosis

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Sarkar, A., Lu, L. L., Han, J., & Alter, G. (2020). An integrated electrical enzyme-linked immunosorbent assay (E2LISA) for point-of-care diagnosis of tuberculosis using glycan biomarkers. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 5-6). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.