An integrated functional genomic analysis identifies the antitumorigenic mechanism of action for PPARγ in lung cancer cells

Rahul K. Kollipara, Ralf Kittler

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Integrating the analysis of the cistrome of a transcription factor by ChIP-Seq with the study of its transcriptional output by microarray or RNA-Seq analysis is a powerful approach to elucidate the genomic functions of a transcription factor. Recently, we employed this approach to determine the mechanism of action by which the nuclear receptor PPARγ elicits its antitumorigenic effects in lung cancer cells upon activation by TZDs (1). Here we describe in detail the design, contents and quality controls for the gene expression and cistrome analyses associated with our study published in Cell Metabolism in 2014.

Original languageEnglish (US)
Pages (from-to)80-86
Number of pages7
JournalGenomics Data
Volume3
DOIs
StatePublished - Mar 1 2015

Keywords

  • Bioinformatics
  • ChIP-Seq
  • Gene expression
  • Lung cancer
  • Microarray

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Genetics

Fingerprint Dive into the research topics of 'An integrated functional genomic analysis identifies the antitumorigenic mechanism of action for PPARγ in lung cancer cells'. Together they form a unique fingerprint.

  • Cite this