An intimate collaboration between peroxisomes and lipid bodies

Derk Binns, Tom Januszewski, Yue Chen, Justin Hill, Vladislav S. Markin, Yingming Zhao, Christopher Gilpin, Kent D. Chapman, Richard G W Anderson, Joel M. Goodman

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

Although peroxisomes oxidize lipids, the metabolism of lipid bodies and peroxisomes is thought to be largely uncoupled from one another. In this study, using oleic acid-cultured Saccharomyces cerevisiae as a model system, we provide evidence that lipid bodies and peroxisomes have a close physiological relationship. Peroxisomes adhere stably to lipid bodies, and they can even extend processes into lipid body cores. Biochemical experiments and proteomic analysis of the purified lipid bodies suggest that these processes are limited to enzymes of fatty acid β oxidation. Peroxisomes that are unable to oxidize fatty acids promote novel structures within lipid bodies ("gnarls"), which may be organized arrays of accumulated free fatty acids. However, gnarls are suppressed, and fatty acids are not accumulated in the absence of peroxisomal membranes. Our results suggest that the extensive physical contact between peroxisomes and lipid bodies promotes the coupling of lipolysis within lipid bodies with peroxisomal fatty acid oxidation.

Original languageEnglish (US)
Pages (from-to)719-731
Number of pages13
JournalJournal of Cell Biology
Volume173
Issue number5
DOIs
StatePublished - Jun 5 2006

Fingerprint

Peroxisomes
Fatty Acids
Lipid Droplets
Lipolysis
Oleic Acid
Lipid Metabolism
Nonesterified Fatty Acids
Proteomics
Saccharomyces cerevisiae
Membranes
Enzymes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Binns, D., Januszewski, T., Chen, Y., Hill, J., Markin, V. S., Zhao, Y., ... Goodman, J. M. (2006). An intimate collaboration between peroxisomes and lipid bodies. Journal of Cell Biology, 173(5), 719-731. https://doi.org/10.1083/jcb.200511125

An intimate collaboration between peroxisomes and lipid bodies. / Binns, Derk; Januszewski, Tom; Chen, Yue; Hill, Justin; Markin, Vladislav S.; Zhao, Yingming; Gilpin, Christopher; Chapman, Kent D.; Anderson, Richard G W; Goodman, Joel M.

In: Journal of Cell Biology, Vol. 173, No. 5, 05.06.2006, p. 719-731.

Research output: Contribution to journalArticle

Binns, D, Januszewski, T, Chen, Y, Hill, J, Markin, VS, Zhao, Y, Gilpin, C, Chapman, KD, Anderson, RGW & Goodman, JM 2006, 'An intimate collaboration between peroxisomes and lipid bodies', Journal of Cell Biology, vol. 173, no. 5, pp. 719-731. https://doi.org/10.1083/jcb.200511125
Binns D, Januszewski T, Chen Y, Hill J, Markin VS, Zhao Y et al. An intimate collaboration between peroxisomes and lipid bodies. Journal of Cell Biology. 2006 Jun 5;173(5):719-731. https://doi.org/10.1083/jcb.200511125
Binns, Derk ; Januszewski, Tom ; Chen, Yue ; Hill, Justin ; Markin, Vladislav S. ; Zhao, Yingming ; Gilpin, Christopher ; Chapman, Kent D. ; Anderson, Richard G W ; Goodman, Joel M. / An intimate collaboration between peroxisomes and lipid bodies. In: Journal of Cell Biology. 2006 ; Vol. 173, No. 5. pp. 719-731.
@article{7adb9e075a9e43a79fe724e384d14156,
title = "An intimate collaboration between peroxisomes and lipid bodies",
abstract = "Although peroxisomes oxidize lipids, the metabolism of lipid bodies and peroxisomes is thought to be largely uncoupled from one another. In this study, using oleic acid-cultured Saccharomyces cerevisiae as a model system, we provide evidence that lipid bodies and peroxisomes have a close physiological relationship. Peroxisomes adhere stably to lipid bodies, and they can even extend processes into lipid body cores. Biochemical experiments and proteomic analysis of the purified lipid bodies suggest that these processes are limited to enzymes of fatty acid β oxidation. Peroxisomes that are unable to oxidize fatty acids promote novel structures within lipid bodies ({"}gnarls{"}), which may be organized arrays of accumulated free fatty acids. However, gnarls are suppressed, and fatty acids are not accumulated in the absence of peroxisomal membranes. Our results suggest that the extensive physical contact between peroxisomes and lipid bodies promotes the coupling of lipolysis within lipid bodies with peroxisomal fatty acid oxidation.",
author = "Derk Binns and Tom Januszewski and Yue Chen and Justin Hill and Markin, {Vladislav S.} and Yingming Zhao and Christopher Gilpin and Chapman, {Kent D.} and Anderson, {Richard G W} and Goodman, {Joel M.}",
year = "2006",
month = "6",
day = "5",
doi = "10.1083/jcb.200511125",
language = "English (US)",
volume = "173",
pages = "719--731",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - An intimate collaboration between peroxisomes and lipid bodies

AU - Binns, Derk

AU - Januszewski, Tom

AU - Chen, Yue

AU - Hill, Justin

AU - Markin, Vladislav S.

AU - Zhao, Yingming

AU - Gilpin, Christopher

AU - Chapman, Kent D.

AU - Anderson, Richard G W

AU - Goodman, Joel M.

PY - 2006/6/5

Y1 - 2006/6/5

N2 - Although peroxisomes oxidize lipids, the metabolism of lipid bodies and peroxisomes is thought to be largely uncoupled from one another. In this study, using oleic acid-cultured Saccharomyces cerevisiae as a model system, we provide evidence that lipid bodies and peroxisomes have a close physiological relationship. Peroxisomes adhere stably to lipid bodies, and they can even extend processes into lipid body cores. Biochemical experiments and proteomic analysis of the purified lipid bodies suggest that these processes are limited to enzymes of fatty acid β oxidation. Peroxisomes that are unable to oxidize fatty acids promote novel structures within lipid bodies ("gnarls"), which may be organized arrays of accumulated free fatty acids. However, gnarls are suppressed, and fatty acids are not accumulated in the absence of peroxisomal membranes. Our results suggest that the extensive physical contact between peroxisomes and lipid bodies promotes the coupling of lipolysis within lipid bodies with peroxisomal fatty acid oxidation.

AB - Although peroxisomes oxidize lipids, the metabolism of lipid bodies and peroxisomes is thought to be largely uncoupled from one another. In this study, using oleic acid-cultured Saccharomyces cerevisiae as a model system, we provide evidence that lipid bodies and peroxisomes have a close physiological relationship. Peroxisomes adhere stably to lipid bodies, and they can even extend processes into lipid body cores. Biochemical experiments and proteomic analysis of the purified lipid bodies suggest that these processes are limited to enzymes of fatty acid β oxidation. Peroxisomes that are unable to oxidize fatty acids promote novel structures within lipid bodies ("gnarls"), which may be organized arrays of accumulated free fatty acids. However, gnarls are suppressed, and fatty acids are not accumulated in the absence of peroxisomal membranes. Our results suggest that the extensive physical contact between peroxisomes and lipid bodies promotes the coupling of lipolysis within lipid bodies with peroxisomal fatty acid oxidation.

UR - http://www.scopus.com/inward/record.url?scp=33747380991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747380991&partnerID=8YFLogxK

U2 - 10.1083/jcb.200511125

DO - 10.1083/jcb.200511125

M3 - Article

VL - 173

SP - 719

EP - 731

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 5

ER -