TY - JOUR
T1 - An intimate collaboration between peroxisomes and lipid bodies
AU - Binns, Derk
AU - Januszewski, Tom
AU - Chen, Yue
AU - Hill, Justin
AU - Markin, Vladislav S.
AU - Zhao, Yingming
AU - Gilpin, Christopher
AU - Chapman, Kent D.
AU - Anderson, Richard G W
AU - Goodman, Joel M.
PY - 2006/6/5
Y1 - 2006/6/5
N2 - Although peroxisomes oxidize lipids, the metabolism of lipid bodies and peroxisomes is thought to be largely uncoupled from one another. In this study, using oleic acid-cultured Saccharomyces cerevisiae as a model system, we provide evidence that lipid bodies and peroxisomes have a close physiological relationship. Peroxisomes adhere stably to lipid bodies, and they can even extend processes into lipid body cores. Biochemical experiments and proteomic analysis of the purified lipid bodies suggest that these processes are limited to enzymes of fatty acid β oxidation. Peroxisomes that are unable to oxidize fatty acids promote novel structures within lipid bodies ("gnarls"), which may be organized arrays of accumulated free fatty acids. However, gnarls are suppressed, and fatty acids are not accumulated in the absence of peroxisomal membranes. Our results suggest that the extensive physical contact between peroxisomes and lipid bodies promotes the coupling of lipolysis within lipid bodies with peroxisomal fatty acid oxidation.
AB - Although peroxisomes oxidize lipids, the metabolism of lipid bodies and peroxisomes is thought to be largely uncoupled from one another. In this study, using oleic acid-cultured Saccharomyces cerevisiae as a model system, we provide evidence that lipid bodies and peroxisomes have a close physiological relationship. Peroxisomes adhere stably to lipid bodies, and they can even extend processes into lipid body cores. Biochemical experiments and proteomic analysis of the purified lipid bodies suggest that these processes are limited to enzymes of fatty acid β oxidation. Peroxisomes that are unable to oxidize fatty acids promote novel structures within lipid bodies ("gnarls"), which may be organized arrays of accumulated free fatty acids. However, gnarls are suppressed, and fatty acids are not accumulated in the absence of peroxisomal membranes. Our results suggest that the extensive physical contact between peroxisomes and lipid bodies promotes the coupling of lipolysis within lipid bodies with peroxisomal fatty acid oxidation.
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U2 - 10.1083/jcb.200511125
DO - 10.1083/jcb.200511125
M3 - Article
C2 - 16735577
AN - SCOPUS:33747380991
SN - 0021-9525
VL - 173
SP - 719
EP - 731
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -