An intimate collaboration between peroxisomes and lipid bodies

Derk Binns, Tom Januszewski, Yue Chen, Justin Hill, Vladislav S. Markin, Yingming Zhao, Christopher Gilpin, Kent D. Chapman, Richard G W Anderson, Joel M. Goodman

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224 Scopus citations

Abstract

Although peroxisomes oxidize lipids, the metabolism of lipid bodies and peroxisomes is thought to be largely uncoupled from one another. In this study, using oleic acid-cultured Saccharomyces cerevisiae as a model system, we provide evidence that lipid bodies and peroxisomes have a close physiological relationship. Peroxisomes adhere stably to lipid bodies, and they can even extend processes into lipid body cores. Biochemical experiments and proteomic analysis of the purified lipid bodies suggest that these processes are limited to enzymes of fatty acid β oxidation. Peroxisomes that are unable to oxidize fatty acids promote novel structures within lipid bodies ("gnarls"), which may be organized arrays of accumulated free fatty acids. However, gnarls are suppressed, and fatty acids are not accumulated in the absence of peroxisomal membranes. Our results suggest that the extensive physical contact between peroxisomes and lipid bodies promotes the coupling of lipolysis within lipid bodies with peroxisomal fatty acid oxidation.

Original languageEnglish (US)
Pages (from-to)719-731
Number of pages13
JournalJournal of Cell Biology
Volume173
Issue number5
DOIs
StatePublished - Jun 5 2006

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ASJC Scopus subject areas

  • Cell Biology

Cite this

Binns, D., Januszewski, T., Chen, Y., Hill, J., Markin, V. S., Zhao, Y., Gilpin, C., Chapman, K. D., Anderson, R. G. W., & Goodman, J. M. (2006). An intimate collaboration between peroxisomes and lipid bodies. Journal of Cell Biology, 173(5), 719-731. https://doi.org/10.1083/jcb.200511125