An intrinsically disordered peptide from ebola virus VP35 controls viral RNA synthesis by modulating nucleoprotein-RNA interactions

Daisy W. Leung, Dominika Borek, Priya Luthra, Jennifer M. Binning, Manu Anantpadma, Gai Liu, Ian B. Harvey, Zhaoming Su, Ariel Endlich-Frazier, Juanli Pan, Reed S. Shabman, Wah Chiu, Robert A. Davey, Zbyszek Otwinowski, Christopher F. Basler, Gaya K. Amarasinghe

Research output: Contribution to journalArticle

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Abstract

During viral RNA synthesis, Ebola virus (EBOV) nucleoprotein (NP) alternates between an RNA-template-bound form and a template-free form to provide the viral polymerase access to the RNA template. In addition, newly synthesized NP must be prevented from indiscriminately binding to noncognate RNAs. Here, we investigate the molecular bases for these critical processes. We identify an intrinsically disordered peptide derived from EBOV VP35 (NPBP, residues 20-48) that binds NP with high affinity and specificity, inhibits NP oligomerization, and releases RNA from NP-RNA complexes invitro. The structure of the NPBP/δNP<inf>NTD</inf> complex, solved to3.7Å resolution, reveals how NPBP peptide occludes a large surface area that is important for NP-NP and NP-RNA interactions and for viral RNA synthesis. Together, our results identify a highly conserved viral interface that is important for EBOV replication and can be targeted for therapeutic development.

Original languageEnglish (US)
Pages (from-to)376-389
Number of pages14
JournalCell Reports
Volume11
Issue number3
DOIs
StatePublished - Apr 21 2015

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Nucleoproteins
Viral RNA
RNA
Peptides
Ebolavirus
Viruses
Oligomerization
Ebola virus nucleoprotein VP35
Virus Replication

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

An intrinsically disordered peptide from ebola virus VP35 controls viral RNA synthesis by modulating nucleoprotein-RNA interactions. / Leung, Daisy W.; Borek, Dominika; Luthra, Priya; Binning, Jennifer M.; Anantpadma, Manu; Liu, Gai; Harvey, Ian B.; Su, Zhaoming; Endlich-Frazier, Ariel; Pan, Juanli; Shabman, Reed S.; Chiu, Wah; Davey, Robert A.; Otwinowski, Zbyszek; Basler, Christopher F.; Amarasinghe, Gaya K.

In: Cell Reports, Vol. 11, No. 3, 21.04.2015, p. 376-389.

Research output: Contribution to journalArticle

Leung, DW, Borek, D, Luthra, P, Binning, JM, Anantpadma, M, Liu, G, Harvey, IB, Su, Z, Endlich-Frazier, A, Pan, J, Shabman, RS, Chiu, W, Davey, RA, Otwinowski, Z, Basler, CF & Amarasinghe, GK 2015, 'An intrinsically disordered peptide from ebola virus VP35 controls viral RNA synthesis by modulating nucleoprotein-RNA interactions', Cell Reports, vol. 11, no. 3, pp. 376-389. https://doi.org/10.1016/j.celrep.2015.03.034
Leung, Daisy W. ; Borek, Dominika ; Luthra, Priya ; Binning, Jennifer M. ; Anantpadma, Manu ; Liu, Gai ; Harvey, Ian B. ; Su, Zhaoming ; Endlich-Frazier, Ariel ; Pan, Juanli ; Shabman, Reed S. ; Chiu, Wah ; Davey, Robert A. ; Otwinowski, Zbyszek ; Basler, Christopher F. ; Amarasinghe, Gaya K. / An intrinsically disordered peptide from ebola virus VP35 controls viral RNA synthesis by modulating nucleoprotein-RNA interactions. In: Cell Reports. 2015 ; Vol. 11, No. 3. pp. 376-389.
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