An invasive podosome-like structure promotes fusion pore formation during myoblast fusion

Kristin L. Sens, Shiliang Zhang, Peng Jin, Rui Duan, Guofeng Zhang, Fengbao Luo, Lauren Parachini, Elizabeth Chen

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107 Scopus citations

Abstract

Recent studies in Drosophila have implicated actin cytoskeletal remodeling in myoblast fusion, but the cellular mechanisms underlying this process remain poorly understood. Here we show that actin polymerization occurs in an asymmetric and cell type-specific manner between a muscle founder cell and a fusion-competent myoblast (FCM). In the FCM, a dense F-actin-enriched focus forms at the site of fusion, whereas a thin sheath of F-actin is induced along the apposing founder cell membrane. The FCM-specific actin focus invades the apposing founder cell with multiple finger-like protrusions, leading to the formation of a single-channel macro fusion pore between the two muscle cells. Two actin nucleation-promoting factors of the Arp2/3 complex, WASP and Scar, are required for the formation of the F-actin foci, whereas WASP but not Scar promotes efficient foci invasion. Our studies uncover a novel invasive podosome-like structure (PLS) in a developing tissue and reveal a previously unrecognized function of PLSs in facilitating cell membrane juxtaposition and fusion.

Original languageEnglish (US)
Pages (from-to)1013-1027
Number of pages15
JournalJournal of Cell Biology
Volume191
Issue number5
DOIs
StatePublished - Nov 29 2010

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ASJC Scopus subject areas

  • Cell Biology

Cite this

Sens, K. L., Zhang, S., Jin, P., Duan, R., Zhang, G., Luo, F., Parachini, L., & Chen, E. (2010). An invasive podosome-like structure promotes fusion pore formation during myoblast fusion. Journal of Cell Biology, 191(5), 1013-1027. https://doi.org/10.1083/jcb.201006006