An investigation of the effect of radioactive labeling of DNA on excision repair in UV-irradiated human fibroblasts

E. K. Ehmann; E. C. Friedberg

doi:10.1016/S0006-3495(80)85058-2

An investigation of the effect of radioactive labeling of DNA on excision repair in UV-irradiated human fibroblasts

E. K. Ehmann, E. C. Friedberg

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Abstract

Previous studies on the kinetics of thymine dimer excision and unscheduled DNA synthesis in UV-irradiated human fibroblasts showed a significant discrepancy in these two parameters (Ehmann et al., 1978. Biophys. J. 22: 249). In the present study we have investigated the effect of the level of the radioactive isotope used for labeling cells on the kinetics of a parameter that directly measures thymine dimer excision. We find no significant differences in the kinetics of this parameter in cells lightly or heavily labeled with radioactive thymidine.

Original language	English (US)
Pages (from-to)	285-291
Number of pages	7
Journal	Biophysical journal
Volume	31
Issue number	2
DOIs	https://doi.org/10.1016/S0006-3495(80)85058-2
State	Published - 1980

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Biophysics

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10.1016/S0006-3495(80)85058-2

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title = "An investigation of the effect of radioactive labeling of DNA on excision repair in UV-irradiated human fibroblasts",

abstract = "Previous studies on the kinetics of thymine dimer excision and unscheduled DNA synthesis in UV-irradiated human fibroblasts showed a significant discrepancy in these two parameters (Ehmann et al., 1978. Biophys. J. 22: 249). In the present study we have investigated the effect of the level of the radioactive isotope used for labeling cells on the kinetics of a parameter that directly measures thymine dimer excision. We find no significant differences in the kinetics of this parameter in cells lightly or heavily labeled with radioactive thymidine.",

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AU - Ehmann, E. K.

AU - Friedberg, E. C.

PY - 1980

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AB - Previous studies on the kinetics of thymine dimer excision and unscheduled DNA synthesis in UV-irradiated human fibroblasts showed a significant discrepancy in these two parameters (Ehmann et al., 1978. Biophys. J. 22: 249). In the present study we have investigated the effect of the level of the radioactive isotope used for labeling cells on the kinetics of a parameter that directly measures thymine dimer excision. We find no significant differences in the kinetics of this parameter in cells lightly or heavily labeled with radioactive thymidine.

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An investigation of the effect of radioactive labeling of DNA on excision repair in UV-irradiated human fibroblasts

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