An isolated pool of vesicles recycles at rest and drives spontaneous neurotransmission

Yildirim Sara, Tuhin Virmani, Ferenc Deák, Xinran Liu, Ege T. Kavalali

Research output: Contribution to journalArticle

276 Citations (Scopus)

Abstract

Spontaneous synaptic vesicle fusion is a common property of all synapses. To trace the origin of spontaneously fused vesicles in hippocampal synapses, we tagged vesicles with fluorescent styryl dyes, antibodies against synaptotagmin-1, or horseradish peroxidase. We could show that synaptic vesicles recycle at rest, and after spontaneous exo-endocytosis, they populate a reluctantly releasable pool of limited size. Interestingly, vesicles in this spontaneously labeled pool were more likely to re-fuse spontaneously compared to vesicles labeled with activity. We found that blocking vesicle refilling at rest selectively depleted neurotransmitter from spontaneously fusing vesicles without significantly altering evoked transmission. Furthermore, in the absence of the vesicle SNARE protein synaptobrevin (VAMP), activity-dependent and spontaneously recycling vesicles could mix, suggesting a role for synaptobrevin in the separation of the two pools. Taken together these results suggest that spontaneously recycling vesicles and activity-dependent recycling vesicles originate from distinct pools with limited cross-talk with each other.

Original languageEnglish (US)
Pages (from-to)563-573
Number of pages11
JournalNeuron
Volume45
Issue number4
DOIs
StatePublished - Feb 17 2005

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R-SNARE Proteins
Synaptic Vesicles
Synaptic Transmission
Synapses
Synaptotagmin I
SNARE Proteins
Horseradish Peroxidase
Endocytosis
Fluorescent Dyes
Neurotransmitter Agents
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)

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An isolated pool of vesicles recycles at rest and drives spontaneous neurotransmission. / Sara, Yildirim; Virmani, Tuhin; Deák, Ferenc; Liu, Xinran; Kavalali, Ege T.

In: Neuron, Vol. 45, No. 4, 17.02.2005, p. 563-573.

Research output: Contribution to journalArticle

Sara, Y, Virmani, T, Deák, F, Liu, X & Kavalali, ET 2005, 'An isolated pool of vesicles recycles at rest and drives spontaneous neurotransmission', Neuron, vol. 45, no. 4, pp. 563-573. https://doi.org/10.1016/j.neuron.2004.12.056
Sara, Yildirim ; Virmani, Tuhin ; Deák, Ferenc ; Liu, Xinran ; Kavalali, Ege T. / An isolated pool of vesicles recycles at rest and drives spontaneous neurotransmission. In: Neuron. 2005 ; Vol. 45, No. 4. pp. 563-573.
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