An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression

Koichi Ogami, Patricia Richard, Yaqiong Chen, Mainul Hoque, Wencheng Li, James J. Moresco, John R. Yates, Bin Tian, James L. Manley

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Many long noncoding RNAs (lncRNAs) are unstable and rapidly degraded in the nucleus by the nuclear exosome. An exosome adaptor complex called NEXT (nuclear exosome targeting) functions to facilitate turnover of some of these lncRNAs. Here we show that knockdown of one NEXT subunit, Mtr4, but neither of the other two subunits, resulted in accumulation of two types of lncRNAs: prematurely terminated RNAs (ptRNAs) and upstream antisense RNAs (uaRNAs). This suggested a NEXT-independent Mtr4 function, and, consistent with this, we isolated a distinct complex containing Mtr4 and the zinc finger protein ZFC3H1. Strikingly, knockdown of either protein not only increased pt/uaRNA levels but also led to their accumulation in the cytoplasm. Furthermore, all pt/uaRNAs examined associated with active ribosomes, but, paradoxically, this correlated with a global reduction in heavy polysomes and overall repression of translation. Our findings highlight a critical role for Mtr4/ZFC3H1 in nuclear surveillance of naturally unstable lncRNAs to prevent their accumulation, transport to the cytoplasm, and resultant disruption of protein synthesis.

Original languageEnglish (US)
Pages (from-to)1257-1271
Number of pages15
JournalGenes and Development
Volume31
Issue number12
DOIs
StatePublished - Jun 15 2017
Externally publishedYes

Fingerprint

Exosomes
Nuclear RNA
Long Noncoding RNA
Antisense RNA
Cytoplasm
Proteins
Polyribosomes
Zinc Fingers
Ribosomes
RNA

Keywords

  • Exosome
  • lncRNA
  • Mtr4
  • Polyadenylation
  • ZFC3H1

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. / Ogami, Koichi; Richard, Patricia; Chen, Yaqiong; Hoque, Mainul; Li, Wencheng; Moresco, James J.; Yates, John R.; Tian, Bin; Manley, James L.

In: Genes and Development, Vol. 31, No. 12, 15.06.2017, p. 1257-1271.

Research output: Contribution to journalArticle

Ogami, Koichi ; Richard, Patricia ; Chen, Yaqiong ; Hoque, Mainul ; Li, Wencheng ; Moresco, James J. ; Yates, John R. ; Tian, Bin ; Manley, James L. / An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. In: Genes and Development. 2017 ; Vol. 31, No. 12. pp. 1257-1271.
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