An oral essential amino acid-carbohydrate supplement enhances muscle protein anabolism after resistance exercise

Blake B. Rasmussen, Kevin D. Tipton, Sharon L. Miller, Steven E. Wolf, Robert R. Wolfe

Research output: Contribution to journalArticle

367 Scopus citations

Abstract

This study was designed to determine the response of muscle protein to the bolus ingestion of a drink containing essential amino acids and carbohydrate after resistance exercise. Six subjects (3 men, 3 women) randomly consumed a treatment drink (6 g essential amino acids, 35 g sucrose) or a flavored placebo drink 1 h or 3 h after a bout of resistance exercise on two separate occasions. We used a three-compartment model for determination of leg muscle protein kinetics. The model involves the infusion of ring- 2H5-phenylalanine, femoral arterial and venous blood sampling, and muscle biopsies. Phenylalanine net balance and muscle protein synthesis were significantly increased above the predrink and corresponding placebo value (P < 0.05) when the drink was taken 1 or 3 h after exercise but not when the placebo was ingested at 1 or 3 h. The response to the amino acid-carbohydrate drink produced similar anabolic responses at 1 and 3 h. Muscle protein breakdown did not change in response to the drink. We conclude that essential amino acids with carbohydrates stimulate muscle protein anabolism by increasing muscle protein synthesis when ingested 1 or 3 h after resistance exercise.

Original languageEnglish (US)
Pages (from-to)386-392
Number of pages7
JournalJournal of applied physiology
Volume88
Issue number2
StatePublished - Feb 1 2000

Keywords

  • Muscle protein synthesis
  • Nutritional supplementation
  • Stable isotopes

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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    Rasmussen, B. B., Tipton, K. D., Miller, S. L., Wolf, S. E., & Wolfe, R. R. (2000). An oral essential amino acid-carbohydrate supplement enhances muscle protein anabolism after resistance exercise. Journal of applied physiology, 88(2), 386-392.