An rfuABCD-like operon and its relationship to riboflavin utilization and mammalian infectivity by Borrelia burgdorferi

Matthew K. Muramatsu, Jianli Zhou, Bryna L. Fitzgerald, Ranjit K. Deka, John T. Belisle, Michael V. Norgard

Research output: Contribution to journalArticlepeer-review

Abstract

Riboflavin is an essential micronutrient, but its transport and utilization have remained largely understudied among pathogenic spirochetes. Here, we show that Borrelia burgdorferi, the zoonotic spirochete that causes Lyme disease, is able to import riboflavin via products of its rfuABCD-like operon as well as synthesize flavin mononucleotide and flavin adenine dinucleotide despite lacking canonical genes for their synthesis. Additionally, a mutant deficient in the rfuABCD-like operon is resistant to the antimicrobial effect of roseoflavin, a natural riboflavin analog, and is attenuated in a murine model of Lyme borreliosis. Our combined results indicate not only that are riboflavin and the maintenance of flavin pools essential for B. burgdorferi growth but also that flavin utilization and its downstream products (e.g., flavoproteins) may play a more prominent role in B. burgdorferi pathogenesis than previously appreciated.

Original languageEnglish (US)
Article numbere00307-21
JournalInfection and immunity
Volume89
Issue number10
DOIs
StatePublished - Oct 2021

Keywords

  • Borrelia burgdorferi
  • Lyme disease
  • RfuABCD
  • Riboflavin
  • Roseoflavin

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'An rfuABCD-like operon and its relationship to riboflavin utilization and mammalian infectivity by Borrelia burgdorferi'. Together they form a unique fingerprint.

Cite this