An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region promoters

R. C. Conaway, J. Weliky Conaway

Research output: Contribution to journalArticlepeer-review

92 Scopus citations

Abstract

A transcription factor required for synthesis of accurately initiated run-off transcripts by RNA polymerase II has been purified and shown to have an associated DNA-dependent ATPase (dATPase) activity that is strongly stimulated by the TATA region of promoters. This transcription factor, designated δ, was purified more than 3000-fold from extracts of crude rat liver nuclei and has a native molecular mass of approximately 230 kDa. DNA-dependent ATPase (dATPase) and transcription activities copurify when δ is analyzed by hydrophobic interaction and ion-exchange HPLC, arguing that transcription factor δ possesses an ATPase (dATPase) activity. ATPase (dATPase) is specific for adenine nucleotides; ATP dATP, but not CTP, UTP, or GTP, are hydrolyzed. ATPase (dATPase) is stimulated by both double-stranded and single-stranded DNAs, including pUC18, ssM13, and poly(dT); however, DNA fragments containing the TATA region of either the adenovirus 2 major late or mouse interleukin 3 promoters stimulate ATPase as much as 10-fold more effectively than DNA fragments containing nonpromoter sequences. These data suggest the intriguing possibility that δ plays a critical role in the ATP (dATP)-dependent activation of run-off transcription through a direct interaction with the TATA region of promoters.

Original languageEnglish (US)
Pages (from-to)7356-7360
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number19
DOIs
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • General

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