Analyses of Streptomyces coelicolor inner membrane proteome by multidimentional protein identification technology

Xuan Ming Shi, Yuan Ming Luo, Gui Feng Zhang, Zhi Guo Su, Yu Bi Huang, Ke Qian Yang

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Streptomyces coelicolor is the model species among streptomycetes. Until now, proteomic analyses of S. coelicolor have been conducted using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry method, few integral membrane proteins were identified due to the hydrophobic and low-abundance nature of these proteins. In this work, 154 possible inner membrane proteins from S. coelicolor were identified using high pH-proteinase K sample preparation method and multidimensional protein identification technology, among them 44 are integral membrane proteins containing at least one transmembrane domain, most peptides and their corresponding proteins were identified experimentally for the first time.

Original languageEnglish (US)
Pages (from-to)814-819
Number of pages6
JournalSheng wu gong cheng xue bao = Chinese journal of biotechnology
Volume21
Issue number5
StatePublished - Sep 2005

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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