Analysis of acquisition of Pseudomonas aeruginosa gastrointestinal mucosal colonization and horizontal transmission in a murine model

Akinobu Kamei, Andrew Y. Koh, Mihaela Gadjeva, Gregory P. Priebe, Stephen Lory, Gerald B. Pier

Research output: Contribution to journalArticle

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Abstract

Background. Laboratory systems to study bacterial transmission and mucosal colonization leading to infection have not been utilized. Methods. We determined whether transmission of various strains of Pseudomonas aeruginosa among individual mice could occur and investigated the properties of such strains in establishing gastrointestinal (GI) mucosal colonization as well as in disseminating systemically after induction of neutropenia. Results. P aeruginosa isolates associated with epidemic spread among patients with cystic fibrosis (CF) readily established GI colonization at higher levels than did strains associated with acute infections in patients without CF, and they outcompeted these strains. Colonization was associated with resistance to bile salts. However, epidemic CF isolates did not disseminate after induction of neutropenia and did not induce as much mucosal pathology as did strains that were capable of disseminating. Conclusion. Murine models can be used to study P aeruginosa transmission and early colonization, and the properties of these strains associated with their known clinical behaviors are mimicked in this setting.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalJournal of Infectious Diseases
Volume201
Issue number1
DOIs
StatePublished - Jan 1 2010

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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